SSR and ddRADseq genotyping to discriminate and protect an Italian white-type celery (Apium graveolens L.) landrace
Author(s)
Date Issued
February 2026
Type
article
Volume
357
ISSN
0304-4238
Journal
Scientia Horticulturae
Abstract
Celery is an important vegetable crop cultivated in Italy with different biotypes. This study investigated the
genetic distinctiveness of the Italian white-type landrace “Bianco di Sperlonga” (BS). Nineteen celery accessions
were genotyped by SSR and SNP/InDel markers. The collection included 17 white-type accessions, belonging to
the landraces BS (n=12) and “Dorato d’Asti” (SA, n=1), and to commercial lines (n=4). Two black-type accessions,
encompassing the “Nero di Trevi” (NT) landrace and a commercial variety were also included. Eight
SSR markers provided 27 alleles that allowed to distinguish the commercial lines from the landraces and among
the latter, the white- and black-type ones, but failed to differentiated BS and SA white-type landraces. By
contrast, SNPs/InDels identified through ddRADseq analysis discriminated BS from SA populations. BS samples
showed high genetic similarity (≥ 98.5%), also confirmed by clustering, population structure and PCoA analyses.
This result was unexpected given the celery prevalent allogamous nature. Both the SSR and ddRAD datasets
showed lower observed than expected heterozygosity, indicating inbreeding within the BS landrace. This resembles
the genetic structure of prevalently autogamous species. This suggested a loss of genetic load in the BS
landrace, possibly due to strict inbreeding in traditional seed multiplication practices. From the ddRAD dataset,
BS private alleles were identified and two PCR-based markers potentially useful for assessing the BS authenticity
and traceability were developed and successfully validated. Overall, the results supported the purposed derivation
of BS from the SA landrace and provide valuable molecular tools for assessing BS genetic distinctiveness
and ensuring traceability.
File(s)![Thumbnail Image]()
Name
2026 - Farinon et al. Celery Sperlonga.pdf
Size
7.07 MB
Format
Adobe PDF
Checksum (MD5)
64d8ab2db9511b41e3b9ec189ef445c5