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http://hdl.handle.net/2067/51730
Title: | De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites | Authors: | Palomba, Marialetizia Libro, Pietro Di Martino, Jessica Roca-Geronès, Xavier Macali, Armando Castrignanò, Tiziana Canestrelli, Daniele Mattiucci, Simonetta |
Journal: | SCIENTIFIC DATA | Issue Date: | 2023 | Abstract: | Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus representing compelling systems for the study of thermal adaptation. The Antarctic anisakid Contracaecum osculatum sp. D is a marine parasite able to survive and thrive both at very cold and warm temperatures within the environment and its hosts. Here, a de novo transcriptome of C. osculatum sp. D was generated for the first time, by performing RNA-Seq experiments on a set of individuals exposed to temperatures experienced by the nematode during its life cycle. The analysis generated 425,954,724 reads, which were assembled and then annotated. The high-quality assembly was validated, achieving over 88% mapping against the transcriptome. The transcriptome of this parasite will represent a valuable genomic resource for future studies aimed at disentangling the genomic architecture of thermal tolerance and metabolic pathways related to temperature stress. |
URI: | http://hdl.handle.net/2067/51730 | ISSN: | 2052-4463 | DOI: | 10.1038/s41597-023-02591-4 |
Appears in Collections: | A1. Articolo in rivista |
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s41597-023-02591-4.pdf | 1.8 MB | Adobe PDF | Request a copy |
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