Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/51154
Title: High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis
Authors: Turco, Silvia 
Russo, Simone
Pietrucci, Daniele 
Filippi, Anita
Milanesi, Marco 
Luzzago, Camilla
Garbarino, Chiara
Palladini, Giorgia
Chillemi, Giovanni 
Ricchi, Matteo
Journal: FRONTIERS IN VETERINARY SCIENCE 
Issue Date: 2024
Abstract: 
Mycobacterium avium subsp. paratuberculosis (MAP) is the aetiological agent of paratuberculosis (Johne's disease) in both domestic and wild ruminants. In the present study, using a whole-genome sequence (WGS) approach, we investigated the genetic diversity of 15 Mycobacterium avium field strains isolated in the last 10 years from red deer inhabiting the Stelvio National Park and affected by paratuberculosis. Combining de novo assembly and a reference-based method, followed by a pangenome analysis, we highlight a very close relationship among 13 MAP field isolates, suggesting that a single infecting event occurred in this population. Moreover, two isolates have been classified as Mycobacterium avium subsp. hominissuis, distinct from the other MAPs under comparison but close to each other. This is the first time that this subspecies has been found in Italy in samples without evident epidemiological correlations, having been isolated in two different locations of the Stelvio National Park and in different years. Our study highlights the importance of a multidisciplinary approach incorporating molecular epidemiology and ecology into traditional infectious disease knowledge in order to investigate the nature of infectious disease in wildlife populations.
URI: http://hdl.handle.net/2067/51154
ISSN: 2297-1769
DOI: 10.3389/fvets.2024.1301667
Rights: CC0 1.0 Universal
Appears in Collections:A1. Articolo in rivista

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