Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/50877
Title: De novo transcriptome assembly and annotation for gene discovery in Salamandra salamandra at the larval stage
Authors: Libro, Pietro
Chiocchio, Andrea
De Rysky, Erika
Di Martino, Jessica
Bisconti, Roberta 
Castrignano', Tiziana 
Canestrelli, Daniele 
Journal: SCIENTIFIC DATA 
Issue Date: 2023
Abstract: 
Dispersal is a key process in ecology and evolutionary biology, as it shapes biodiversity patterns over space and time. Attitude to disperse is unevenly distributed among individuals within populations, and that individual personality can have pivotal roles in the shaping of this attitude. Here, we assembled and annotated the first de novo transcriptome of the head tissues of Salamandra salamandra from individuals, representative of distinct behavioral profiles. We obtained 1,153,432,918 reads, which were successfully assembled and annotated. The high-quality of the assembly was confirmed by three assembly validators. The alignment of contigs against the de novo transcriptome led to a mapping percentage higher than 94%. The homology annotation with DIAMOND led to 153,048 (blastx) and 95,942 (blastp) shared contigs, annotated on NR, Swiss-Prot and TrEMBL. The domain and site protein prediction led to 9850 GO-annotated contigs. This de novo transcriptome represents reliable reference for comparative gene expression studies between alternative behavioral types, for comparative gene expression studies within Salamandra, and for whole transcriptome and proteome studies in amphibians.
URI: http://hdl.handle.net/2067/50877
ISSN: 2052-4463
DOI: 10.1038/s41597-023-02217-9
Rights: Attribution 4.0 International
Appears in Collections:A1. Articolo in rivista

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