Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2067/50726
Titolo: High clonality of Mycobacterium avium subsp. paratuberculosis field isolates from red deer revealed by two different methodological approaches of comparative genomic analysis
Autori: Turco, Silvia
Russo, Simone
Pietrucci, Daniele
Filippi, Anita
Milanesi, Marco 
Luzzago, Camilla
Garbarino, Chiara
Palladini, Giorgia
Chillemi, Giovanni 
Ricchi, Matteo
Rivista: FRONTIERS IN VETERINARY SCIENCE 
Data pubblicazione: 2024
Abstract: 
Mycobacterium avium subsp. paratuberculosis (MAP) is the aetiological agent
of paratuberculosis (Johne’s disease) in both domestic and wild ruminants.
In the present study, using a whole-genome sequence (WGS) approach,
we investigated the genetic diversity of 15 Mycobacterium avium field strains
isolated in the last 10 years from red deer inhabiting the Stelvio National Park and
affected by paratuberculosis. Combining de novo assembly and a reference-
based method, followed by a pangenome analysis, we highlight a very close
relationship among 13 MAP field isolates, suggesting that a single infecting
event occurred in this population. Moreover, two isolates have been classified as
Mycobacterium avium subsp. hominissuis, distinct from the other MAPs under
comparison but close to each other. This is the first time that this subspecies
has been found in Italy in samples without evident epidemiological correlations,
having been isolated in two different locations of the Stelvio National Park and
in different years. Our study highlights the importance of a multidisciplinary
approach incorporating molecular epidemiology and ecology into traditional
infectious disease knowledge in order to investigate the nature of infectious
disease in wildlife populations.
URI: http://hdl.handle.net/2067/50726
ISSN: 2297-1769
DOI: 10.3389/fvets.2024.1301667
Diritti: Attribution-NonCommercial-ShareAlike 4.0 International
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