Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/49965
Title: Thermal treatment of magnesium particles in polylactic acid polymer films elicits the expression of osteogenic differentiation markers and lipidome profile remodeling in human adipose stem cells
Authors: Argentati, Chiara
Dominici, Franco
MORENA, FRANCESCO
Rallini, Marco
TORTORELLA, ILARIA
Ferrandez-Montero, ANA
PELLEGRINO, ROBERTO MARIA
FERRARI, BEGONA
EMILIANI, CARLA
LIEBLICH, MARCELA
Torre, Luigi
MARTINO, SABATA
Armentano, Ilaria 
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 
Issue Date: 2022
Abstract: 
The efficacy of polylactic acid (PLA)/Magnesium (Mg)-based materials for driving stem cells toward bone tissue engineering applications requires specific Mg surface properties to modulate the interface of stem cells with the film. Here, we have developed novel PLA/Mg-based composites and explored their osteogenic differentiation potential on human adipose stem cells (hASCs). Mg-particles/polymer interface was improved by two treatments: heating in oxidative atmosphere (TT) and surface modification with a compatibilizer (PEI). Different contents of Mg particles were dispersed in PLA and composite surface and bulk properties, protein adsorption, stem cell-PLA/Mg interactions, osteogenic markers expressions, and lipids composition profile were evaluated. Mg particles were uniformly distributed on the surface and in the bulk PLA polymer. Improved and modulated particle-polymer adhesion was observed in Mg particle-treated composites.

After 21 days in canonical growth culture conditions, hASCs on PLA/MgTT displayed the highest expression of the general osteogenic markers, RUNX2, SSP1, and BGLAP genes, Alkaline Phosphatase, type I Collagen, Osteopontin, and Calcium deposits. Moreover, by LC/MS QTOF mass-spectrophotometry lipidomic analysis, we found in PLA/MgTT-cells, for the first time, a remodeling of the lipid classes composition associated with the osteogenic differentiation. We ascribed these results to MgTT characteristics, which improve Mg availability and composite osteoinductive performance.
URI: http://hdl.handle.net/2067/49965
ISSN: 0141-8130
DOI: https://doi.org/10.1016/j.ijbiomac.2022.11.005
Appears in Collections:A1. Articolo in rivista

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