Semen qualitative parameters and spermatozoon ultrastructure of Phasianus colchicus mongolicus

Abstract

In many European countries, the common pheasant is one of the most popular game bird species. Despite its popularity, little information has been published related to its sperm profile, including the common semen parameters used to characterise the quality of this material. Additionally, the spermatozoon’s ultrastructure has never been characterised, and very few data on reproductive performance in the male bird are available. The aim of the present study was to provide these data and fill these gaps in the literature. Semen was collected by the dorso-abdominal massage technique for pheasants and evaluated during the late spring period (May to mid-June). For the ultrastructure and morphometric descriptions, scanning electron and transmission electron images were obtained. The data show that pheasants produce ejaculates of a smaller volume (average volume = 131 µL) but with a higher sperm concentration compared with roosters. Semen pH values tended towards alkaline values (average pH = 8.3). The general shape of the pheasant spermatozoon resembles that of roosters and turkeys, although a bigger sub-acrosomal space was observed compared with these species. As a consequence, its perforatorium occupies two-thirds of the acrosome. A lower number of mitochondria (20–24) was also detected compared with roosters and turkeys. The pheasant spermatozoon’s dimensions are most similar to those observed in turkeys and guineafowl. The present study provides basic information useful for assisted reproductive techniques and conservation programmes. HIGHLIGHTS Basic information on the common pheasant semen is given. A morphometric description of the common pheasant sperm cell is reported. The characterisation of the sperm ultrastructure by scanning and transmission electron microscopy is shown.