Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/48063
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dc.contributor.authorCostantini, Paolo Eit
dc.contributor.authorVanpouille, Christopheit
dc.contributor.authorFirrincieli, Andreait
dc.contributor.authorCappelletti, Martinait
dc.contributor.authorMargolis, Leonidit
dc.contributor.authorÑahui Palomino, Rogers Ait
dc.date.accessioned2022-06-27T20:19:07Z-
dc.date.available2022-06-27T20:19:07Z-
dc.date.issued2022it
dc.identifier.issn2235-2988it
dc.identifier.urihttp://hdl.handle.net/2067/48063-
dc.description.abstractVaginal microbiota dominated by lactobacilli protects women from sexually transmitted infection, in particular HIV-1. This protection is, in part, mediated by Lactobacillus-released extracellular vesicles (EVs). Here, we investigated whether EVs derived from other Gram-positive bacteria also present in healthy vaginas, in particular Staphylococcus aureus, Gardnerella vaginalis, Enterococcus faecium, and Enterococcus faecalis, can affect vaginal HIV-1 infection. We found that EVs released by these bacteria protect human cervico-vaginal tissues ex vivo and isolated cells from HIV-1 infection by inhibiting HIV-1-cell receptor interactions. This inhibition was associated with a diminished exposure of viral Env by steric hindrance of gp120 or gp120 modification evidenced by the failure of EV-treated virions to bind to nanoparticle-coupled anti-Env antibodies. Furthermore, we found that protein components associated with EV's outer surface are critical for EV-mediated protection from HIV-1 infection since treatment of bacteria-released EVs with proteinase K abolished their anti-HIV-1 effect. We identified numerous EV-associated proteins that may be involved in this protection. The identification of EVs with specific proteins that suppress HIV-1 may lead to the development of novel strategies for the prevention of HIV-1 transmission.it
dc.language.isoengit
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleExtracellular Vesicles Generated by Gram-Positive Bacteria Protect Human Tissues Ex Vivo From HIV-1 Infectionit
dc.typearticle*
dc.identifier.doi10.3389/fcimb.2021.822882it
dc.identifier.pmid35145925it
dc.identifier.scopus2-s2.0-85124274172it
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85124274172it
dc.relation.journalFRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGYit
dc.relation.firstpage822882it
dc.relation.volume11it
dc.description.internationalit
dc.contributor.countryITAit
dc.contributor.countryUSAit
dc.type.miur262*
item.fulltextWith Fulltext-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.journal.journalissn2235-2988-
crisitem.journal.anceE215518-
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