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Title: Human Adipose-Derived Stem Cell-Conditioned Medium Promotes Vascularization of Nanostructured Scaffold Transplanted into Nude Mice
Authors: Barone, Ludovica
Rossi, Federica
Valdatta, Luigi
Cherubino, Mario
Papait, Roberto
Binelli, Giorgio
Romano, Nicla 
Bernardini, Giovanni
Gornati, Rosalba
Issue Date: 2022
Abstract: Several studies have been conducted on the interaction between three-dimensional scaffolds
and mesenchymal stem cells for the regeneration of damaged tissues. Considering that stem cells
do not survive for sufficient time to directly sustain tissue regeneration, it is essential to develop
cell-free systems to be applied in regenerative medicine. In this work, by in vivo experiments, we
established that a collagen-nanostructured scaffold, loaded with a culture medium conditioned with
mesenchymal stem cells derived from adipose tissue (hASC-CM), exerts a synergic positive effect
on angiogenesis, fundamental in tissue regeneration. To this aim, we engrafted athymic BALB-C
nude mice with four different combinations: scaffold alone; scaffold with hASCs; scaffold with
hASC crude protein extract; scaffold with hASC-CM. After their removal, we verified the presence of
blood vessels by optical microscopy and confirmed the vascularization evaluating, by real-time PCR,
several vascular growth factors: CD31, CD34, CD105, ANGPT1, ANGPT2, and CDH5. Our results
showed that blood vessels were absent in the scaffold grafted alone, while all the other systems
appeared vascularized, a finding supported by the over-expression of CD31 and CDH5 mRNA. In
conclusion, our data sustain the capability of hASC-CM to be used as a therapeutic cell-free approach
for damaged tissue regeneration.
ISSN: 2079-4991
DOI: 10.3390/nano12091521
Rights: CC0 1.0 Universal
Appears in Collections:A1. Articolo in rivista

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