Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/47602
Title: | Pyrosequencing reveals the effect of mobilizing agents and lignocellulosic substrate amendment on microbial community composition in a real industrial PAH-polluted soil | Authors: | Lladó, S Covino, S Solanas, A M Petruccioli, Maurizio D'Annibale, Alessandro Viñas, M |
Journal: | JOURNAL OF HAZARDOUS MATERIALS | Issue Date: | 2015 | Abstract: | Bacterial and fungal biodiversity throughout different biostimulation and bioaugmentation treatments applied to an industrial creosote-polluted soil were analyzed by means of polyphasic approach in order to gain insight into the microbial community structure and dynamics. Pyrosequencing data obtained from initial creosote polluted soil (after a biopiling step) revealed that Alpha and Gammaproteobacteria were the most abundant bacterial groups, whereas Fusarium and Scedosporium were the main fungal genera in the contaminated soil. At the end of 60-days laboratory scale bioremediation assays, pyrosequencing and DGGE data showed that (i) major bacterial community shifts were caused by the type of mobilizing agent added to the soil and, to a lesser extent, by the addition of lignocellulosic substrate; and (ii) the presence of the non-ionic surfactant (Brij 30) hampered the proliferation of Actinobacteria (Mycobacteriaceae) and Bacteroidetes (Chitinophagaceae) and, in the absence of lignocellulosic substrate, also impeded polycyclic aromatic hydrocarbons (PAHs) degradation. The results show the importance of implementing bioremediation experiments combined with microbiome assessment to gain insight on the effect of crucial parameters (e.g. use of additives) over the potential functions of complex microbial communities harbored in polluted soils, essential for bioremediation success. |
URI: | http://hdl.handle.net/2067/47602 | ISSN: | 0304-3894 | DOI: | 10.1016/j.jhazmat.2014.08.065 | Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International |
Appears in Collections: | A1. Articolo in rivista |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
HAZMAT-S-14-02908-1.pdf | 1.36 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
5
62
Last Week
0
0
Last month
0
0
checked on Apr 17, 2024
Page view(s)
91
Last Week
0
0
Last month
0
0
checked on Apr 24, 2024
Download(s)
20
checked on Apr 24, 2024
Google ScholarTM
Check
Altmetric
This item is licensed under a Creative Commons License