Molecular markers dataset to assess the genetic diversity of oriental plane trees from historical sites in Lazio (central Italy)

Abstract

The dataset presented in this article is related to the re- search paper titled “Dimensional and genetic characterization of the last oriental plane trees (Platanus orientalis L.) of his- torical sites in Lazio (central Italy)” (Ciaffi et al., 2022). In- deed, the molecular analyses reported in that article con- sisted in a comparison of Italian veteran plane trees with 12 certified accessions of P. orientalis, P. occidentalis and their hy- brids P. acerifolia (4 individuals per species). First, LEAFY gene

analyses allowed identifying 32 P. orientalis and two P. acer- ifolia in four sites of the province of Rome, confirming also that the two representative trees from the two gardens of the province of Viterbo belong to P. orientalis. Second, the use of Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) molecular markers provided useful information regarding the genetic relationships within and among all the historical sites. Owing to the use of SSR and ISSR molecu- lar markers, a dataset of parameters related to the genetic diversity of the same plant material was obtained and pre- sented in this article. For SSR markers, seven loci previously developed for P. occidentalis (Lang, 2010) and two specifi- cally developed for P. orientalis (Rinaldi et al., 2019) were em- ployed. For ISSR markers, DNA samples were amplified with eight primers before used for the determination of genetic stability of micro-propagated plantlets of P. acerifolia (Huang et al., 2009) and for the genetic characterization of plane trees within the formal gardens of Villa Lante of Bagnaia and Palazzo Farnese (Viterbo, Italy) (Ciaffi et al., 2018). To the best of our knowledge, this is the first report on the genetic di- versity data for veteran oriental plane trees within heritage sites, which will offer helpful information for their manage- ment and conservation.