Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/47480
Title: | Effect of wine inhibitors on free pineapple stem bromelain activity in a model wine system | Authors: | Esti, Marco Benucci, Ilaria Liburdi, Katia Garzillo, Anna Maria Vittoria |
Journal: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Issue Date: | 2011 | Abstract: | The influence of potential inhibitors, naturally present in wine, on the activity of stem bromelain was investigated in order to evaluate the applicability of this enzyme for protein stabilization in white wine. Bromelain proteolytic activity was tested against a synthetic substrate (Bz-Phe-Val-Arg-pNA) in a model wine system after adding ethanol, sulfur dioxide (SO(2)), skin, seed, and gallic and ellagic tannins at the average range of their concentration in wine. All the inhibitors of stem bromelain activity tested turned out to be reversible. Ethanol was a competitive inhibitor with a rather limited effect. Gallic and ellagic tannins have no inhibitory effect on stem bromelain activity, while both seed and skin tannins were uncompetitive inhibitors. The strongest inhibition effect was revealed for sulfur dioxide, which was a mixed-type inhibitor for the enzyme activity. This study provides useful information relative to a future biotechnological application of stem bromelain in winemaking. |
URI: | http://hdl.handle.net/2067/47480 | ISSN: | 0021-8561 | DOI: | 10.1021/jf104919v | Rights: | CC0 1.0 Universal |
Appears in Collections: | A1. Articolo in rivista |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Effect_of_wine_inhibitors_on_free_pineapple_stem_bromelain_activity_in_a_model_wine_system (2).pdf | Effect of Wine Inhibitors on Free Pineapple Stem Bromelain Activity in a Model Wine System | 978.74 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
5
27
Last Week
0
0
Last month
0
0
checked on Apr 17, 2024
Page view(s)
46
Last Week
0
0
Last month
0
0
checked on Apr 24, 2024
Download(s)
101
checked on Apr 24, 2024
Google ScholarTM
Check
Altmetric
This item is licensed under a Creative Commons License