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Title: Human micronucleus project: International database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei
Authors: Bonassi, Stefano
Fenech, Michael
Lando, Cecilia
Lin, Yi ping
Ceppi, Marcello
Peter Chang, Wushou
Holland, Nina
Kirsch-Volders, Micheline
Zeiger, E.
Ban, Sadayuki
Barale, Roberto
Bigatti, Maria Paola
Bolognesi, Claudia
Jia, Cao
Di Giorgio, Marina
Ferguson, Lynnette R.
Fucic, Aleksandra
Lima, Omar Garcia
Hrelia, P.
Krishnaja, Ayyathan P.
Lee, Tung Kwang
Migliore, Lucia
Mikhalevich, Ludmilla
Mirkova, Ekaterina
Mosesso, P 
Müller, Wolfgang Ulrich
Odagiri, Youichi
Scarffi, Maria Rosaria
Szabova, Elena
Vorobtsova, Irena
Vral, Anne
Zijno, Andrea
Issue Date: 2001
Micronucleus (MN) expression in peripheral blood lymphocytes is well established as a standard method for monitoring chromosome damage in human populations. The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus (CBMN) assay and participating in the HUMN (HUman MicroNucleus project) international collaborative study are presented. The effects of laboratory protocol, scoring criteria, and host factors on baseline micronucleated binucleate cell (MNC) frequency are evaluated, and a reference range of "normal" values against which future studies may be compared is provided. Primary data from historical records were submitted by 25 laboratories distributed in 16 countries. This resulted in a database of nearly 7000 subjects. Potentially significant differences were present in the methods used by participating laboratories, such as in the type of culture medium, the concentration of cytochalasin-B, the percentage of fetal calf serum, and in the culture method. Differences in criteria for scoring micronuclei were also evident. The overall median MNC frequency in nonexposed (i.e., normal) subjects was 6.5‰ and the interquartile range was between 3 and 12‰. An increase in MNC frequency with age was evident in all but two laboratories. The effect of gender, although not so evident in all databases, was also present, with females having a 19% higher level of MNC frequency (95% confidence interval: 14-24%). Statistical analyses were performed using random-effects models for correlated data. Our best model, which included exposure to genotoxic factors, host factors, methods, and scoring criteria, explained 75% of the total variance, with the largest contribution attributable to laboratory methods. © 2001 Wiley-Liss, Inc.
ISSN: 0893-6692
DOI: 10.1002/1098-2280(2001)37:1<31::AID-EM1004>3.0.CO;2-P
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