Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/46352
Title: Multiple Recombination Events and Strong Purifying Selection at the Origin of SARS-CoV-2 Spike Glycoprotein Increased Correlated Dynamic Movements
Authors: Tagliamonte, Massimiliano S
Abid, Nabil
Borocci, Stefano 
Sangiovanni, Elisa
Ostrov, David A
Kosakovsky Pond, Sergei L
Salemi, Marco
Chillemi, Giovanni 
Mavian, Carla
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date: 2021
Abstract: 
Our evolutionary and structural analyses revealed that the severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) spike gene is a complex mosaic resulting from several recombination events. Additionally, the fixation of variants has mainly been driven by purifying selection, suggesting the presence of conserved structural features. Our dynamic simulations identified two main long-range covariant dynamic movements of the novel glycoprotein, and showed that, as a result of the evolutionary duality, they are preserved. The first movement involves the receptor binding domain with the N-terminal domain and the C-terminal domain 2 and is maintained across human, bat and pangolin coronaviruses. The second is a complex network of long-range dynamics specific to SARS-CoV-2 involving the novel PRRA and the conserved KR*SF cleavage sites, as well as conserved segments in C-terminal domain 3. These movements, essential for host cell binding, are maintained by hinges conserved across human, bat, and pangolin coronaviruses glycoproteins. The hinges, located around Threonine 333 and Proline 527 within the N-terminal domain and C-terminal domain 2, represent candidate targets for the future development of novel pan-coronavirus inhibitors. In summary, we show that while recombination created a new configuration that increased the covariant dynamic movements of the SARS-CoV-2 glycoprotein, negative selection preserved its inter-domain structure throughout evolution in different hosts and inter-species transmissions.
URI: http://hdl.handle.net/2067/46352
ISSN: 1422-0067
DOI: 10.3390/ijms22010080
Rights: Attribution-NonCommercial-ShareAlike 4.0 International
Appears in Collections:A1. Articolo in rivista

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