Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/46276
Title: A metabolic profiling analysis revealed a primary metabolism reprogramming in Arabidopsis glyI4 loss-of-function mutant
Authors: Proietti, Silvia 
Bertini, Laura 
Falconieri, Gaia Salvatore
Baccelli, Ivan
Timperio, Anna Maria 
Caruso, Carla 
Journal: PLANTS 
Issue Date: 2021
Abstract: 
Methylglyoxal (MG) is a cytotoxic compound often produced as a side product of metabolic processes such as glycolysis, lipid peroxidation, and photosynthesis. MG is mainly scavenged by the glyoxalase system, a two-step pathway, in which the coordinate activity of GLYI and GLYII transforms it into D-lactate, releasing GSH. In Arabidopsis thaliana, a member of the GLYI family named GLYI4 has been recently characterized. In glyI4 mutant plants, a general stress phenotype characterized by compromised MG scavenging, accumulation of reactive oxygen species (ROS), stomatal closure, and reduced fitness was observed. In order to shed some light on the impact of gly4 loss-of-function on plant metabolism, we applied a high resolution mass spectrometry-based metabolomic approach to Arabidopsis Col-8 wild type and glyI4 mutant plants. A compound library containing a total of 70 metabolites, differentially synthesized in glyI4 compared to Col-8, was obtained. Pathway analysis of the identified compounds showed that the upregulated pathways are mainly involved in redox reactions and cellular energy maintenance, and those downregulated in plant defense and growth. These results improved our understanding of the impacts of glyI4 loss-of-function on the general reprogramming of the plant’s metabolic landscape as a strategy for surviving under adverse physiological conditions.
URI: http://hdl.handle.net/2067/46276
ISSN: 2223-7747
DOI: 10.3390/plants10112464
Rights: Attribution 3.0 United States
Appears in Collections:A1. Articolo in rivista

Files in This Item:
File Description SizeFormat Existing users please
2021_Plants.pdf1.33 MBAdobe PDF    Request a copy
Show full item record

SCOPUSTM   
Citations 20

8
Last Week
0
Last month
2
checked on Mar 24, 2024

Page view(s)

65
Last Week
0
Last month
0
checked on Mar 27, 2024

Download(s)

3
checked on Mar 27, 2024

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons