Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/46089
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dc.contributor.authorMorena, Francescoit
dc.contributor.authorArgentati, Chiarait
dc.contributor.authorSoccio, Michelinait
dc.contributor.authorBicchi, Ilariait
dc.contributor.authorLuzi, Francescait
dc.contributor.authorTorre, Luigiit
dc.contributor.authorMunari, Andreait
dc.contributor.authorEmiliani, Carlait
dc.contributor.authorGigli, Matteoit
dc.contributor.authorLotti, Nadiait
dc.contributor.authorArmentano, Ilariait
dc.contributor.authorMartino, Sabatait
dc.date.accessioned2021-11-09T14:05:31Z-
dc.date.available2021-11-09T14:05:31Z-
dc.date.issued2020it
dc.identifier.issn1661-6596it
dc.identifier.urihttp://hdl.handle.net/2067/46089-
dc.description.abstractHerein, we present poly(butylene 1,4-cyclohexanedicarboxylate) (PBCE) films characterized by an unpatterned microstructure and a specific hydrophobicity, capable of boosting a drastic cytoskeleton architecture remodeling, culminating with the neuronal-like differentiation of human bone marrow-mesenchymal stem cells (hBM-MSCs). We have used two different filming procedures to prepare the films, solvent casting (PBCE) and compression-moulding (PBCE*). PBCE film had a rough and porous surface with spherulite-like aggregations (Ø = 10-20 μm) and was characterized by a water contact angle = 100°. PBCE* showed a smooth and continuous surface without voids and visible spherulite-like aggregations and was more hydrophobic (WCA = 110°). Both surface characteristics were modulated through the copolymerization of different amounts of ether-oxygen-containing co-units into PBCE chemical structure. We showed that only the surface characteristics of PBCE-solvent-casted films steered hBM-MSCs toward a neuronal-like differentiation. hBM-MSCs lost their canonical mesenchymal morphology, acquired a neuronal polarized shape with a long cell protrusion (≥150 μm), expressed neuron-specific class III β-tubulin and microtubule-associated protein 2 neuronal markers, while nestin, a marker of uncommitted stem cells, was drastically silenced. These events were observed as early as 2-days after cell seeding. Of note, the phenomenon was totally absent on PBCE* film, as hBM-MSCs maintained the mesenchymal shape and behavior and did not express neuronal/glial markers.it
dc.language.isoengit
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.titleUnpatterned Bioactive Poly(Butylene 1,4-Cyclohexanedicarboxylate)-Based Film Fast Induced Neuronal-Like Differentiation of Human Bone Marrow-Mesenchymal Stem Cellsit
dc.typearticle*
dc.identifier.doi10.3390/ijms21239274it
dc.identifier.pmid33291757it
dc.identifier.scopus2-s2.0-85097409959it
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85097409959it
dc.relation.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCESit
dc.relation.firstpage1it
dc.relation.lastpage23it
dc.relation.volume21it
dc.relation.issue23it
dc.description.internationalnoit
dc.contributor.countryITAit
dc.type.miur262*
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.journal.journalissn1661-6596-
crisitem.journal.anceE217024-
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