Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/46078
Title: | Src tyrosine kinase inhibitor and X-rays combined effect on glioblastoma cell lines | Authors: | Torrisi, Filippo Minafra, Luigi Cammarata, Francesco P. Savoca, Gaetano Calvaruso, Marco Vicario, Nunzio Maccari, Laura Pérès, Elodie A. Özçelik, Hayriye Bernaudin, Myriam botta, lorenzo Russo, Giorgio Parenti, Rosalba Valable, Samuel |
Journal: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Issue Date: | 2020 | Abstract: | Glioblastoma (GBM) is one of the most lethal types of tumor due to its high recurrence level in spite of aggressive treatment regimens involving surgery, radiotherapy and chemotherapy. Hypoxia is a feature of GBM, involved in radioresistance, and is known to be at the origin of treatment failure. The aim of this work was to assess the therapeutic potential of a new targeted c-SRC inhibitor molecule, named Si306, in combination with X-rays on the human glioblastoma cell lines, comparing normoxia and hypoxia conditions. For this purpose, the dose modifying factor and oxygen enhancement ratio were calculated to evaluate the Si306 radiosensitizing effect. DNA damage and the repair capability were also studied from the kinetic of γ-H2AX immunodetection. Furthermore, motility processes being supposed to be triggered by hypoxia and irradiation, the role of c-SRC inhibition was also analyzed to evaluate the migration blockage by wound healing assay. Our results showed that inhibition of the c-SRC protein enhances the radiotherapy efficacy both in normoxic and hypoxic conditions. These data open new opportunities for GBM treatment combining radiotherapy with molecularly targeted drugs to overcome radioresistance. |
URI: | http://hdl.handle.net/2067/46078 | ISSN: | 1661-6596 | DOI: | 10.3390/ijms21113917 | Rights: | Attribution-NonCommercial-NoDerivs 3.0 United States |
Appears in Collections: | A1. Articolo in rivista |
Files in This Item:
File | Description | Size | Format | Existing users please |
---|---|---|---|---|
ijms-21-03917.pdf | Article | 5.93 MB | Adobe PDF | Request a copy |
SCOPUSTM
Citations
10
20
Last Week
2
2
Last month
0
0
checked on Mar 24, 2024
Page view(s)
59
Last Week
0
0
Last month
1
1
checked on Mar 27, 2024
Download(s)
3
checked on Mar 27, 2024
Google ScholarTM
Check
Altmetric
This item is licensed under a Creative Commons License