Exchangeable cations and pH drive diversity and functionality of fungal communities in biological soil crusts from coastal sites of Victoria Land, Antarctica

Abstract

Ice-free regions in coastal areas of Victoria Land, Antarctica, are patchily distributed, limited in extent and characterized by a simple vegetation of lichens and mosses, growing only for a short period during the austral summer. These organisms are associated with soil particles and microorganisms (e.g., algae, microfungi and bacteria) to make up biological soil crusts (BSCs), found worldwide in cold and/or arid and semi-arid regions, where plant growth is impaired. Despite BSCs being among the most widespread ecosystems throughout coastal ice-free areas of continental Antarctica, fungal components of these communities have received little focus. Through ITS1 DNA metabarcoding of samples from 17 sites of six different localities from 73 to 77°S, in a distance scale from 29 to 411 km among different sites, we provide insights into the diversity, community composition, and functionality of fungal communities of these peculiar ecosystems, deepening our knowledge on how they are related to different edaphic variables (i.e. chemical properties and texture). Although fungal richness was low (59 ± 27 OTUs per sample), we found numerous previously unsequenced, putatively unknown fungal species representing a great part of the sampled communities. Community composition was spatially auto-correlated and appeared to be driven by site-specific differences in environmental conditions, particularly edaphic factors, such as exchangeable cations and pH. These results are of particular interest, as they give a wide characterization of the parameters determining soil colonization in a such limiting environment, especially in the light of global changes that are expected to deeply modify the conditions of this environment.