Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43701
Title: Ft-ir transflection micro-spectroscopy study on normal human breast cells after exposure to a proton beam
Authors: Ricciardi, Valerio
Portaccio, Marianna
Perna, Giuseppe
Lasalvia, Maria
Capozzi, Vito
Cammarata, Francesco Paolo
Pisciotta, Pietro
Petringa, Giada
Delfino, Ines 
Manti, Lorenzo
Lepore, Maria
Journal: APPLIED SCIENCES 
Issue Date: 2021
Abstract: 
Fourier transform infrared micro-spectroscopy (µ-FT-IR) is nowadays considered a valuable tool for investigating the changes occurring in human cells after exposure to ionizing radiation. Recently, considerable attention has been devoted to the use of this optical technique in the study of cells exposed to proton beams, that are being increasingly adopted in cancer therapy. Different experimental configurations are used for proton irradiation and subsequent spectra acquisition. To facilitate the use of µ-FT-IR, it may be useful to investigate new experimental approaches capable of speeding up and simplifying the irradiation and measurements phases. Here, we propose the use of low-e-substrates slides for cell culture, allowing the irradiation and spectra acquisition in transflection mode in a fast and direct way. In recent years, there has been a wide debate about the validity of these supports, but many researchers agree that the artifacts due to the presence of the electromagnetic standing wave effects are negligible in many practical cases. We investigated human normal breast cells (MCF-10 cell line) fixed immediately after the irradiation with graded proton radiation doses (0, 0.5, 2, and 4 Gy). The spectra obtained in transflection geometry showed characteristics very similar to those present in the spectra acquired in transmission geometry and confirm the validity of the chosen approach. The analysis of spectra indicates the occurrence of significant changes in DNA and lipids components of cells. Modifications in protein secondary structure are also evidenced.
URI: http://hdl.handle.net/2067/43701
ISSN: 2076-3417
DOI: 10.3390/app11020540
Rights: Attribution-NonCommercial-NoDerivs 3.0 United States
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