Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43676
DC FieldValueLanguage
dc.contributor.authorGarcia Molina Maria Doloresit
dc.contributor.authorBotticella Ermelindait
dc.contributor.authorBeleggia Rominait
dc.contributor.authorPalombieri Samuelait
dc.contributor.authorDe Vita Pasqualeit
dc.contributor.authorMasci, Stefaniait
dc.contributor.authorLafiandra, Domenicoit
dc.contributor.authorSestili, Francescoit
dc.date.accessioned2021-10-26T07:54:14Z-
dc.date.available2021-10-26T07:54:14Z-
dc.date.issued2021it
dc.identifier.issn1432-2242it
dc.identifier.urihttp://hdl.handle.net/2067/43676-
dc.description.abstractVitamin A deficiency is a major public health problem that affects numerous countries in the world. As humans are not able to synthesize vitamin A, it must be daily assimilated along with other micro- and macronutrients through the diet. Durum wheat is an important crop for Mediterranean countries and provides a discrete amount of nutrients, such as carbohydrates and proteins, but it is deficient in some essential micronutrients, including provitamin A. In the present work, a targeting induced local lesions in genomes strategy has been undertaken to obtain durum wheat genotypes biofortified in provitamin A. In detail, we focused on the suppression of the β-carotene hydroxylase 1 (HYD1) genes, encoding enzymes involved in the redirection of β-carotene toward the synthesis of the downstream xanthophylls (neoxanthin, violaxanthin and zeaxanthin). Expression analysis of genes involved in carotenoid biosynthesis revealed a reduction of the abundance of HYD1 transcripts greater than 50% in mutant grain compared to the control. The biochemical profiling of carotenoid in the wheat mutant genotypes highlighted a significant increase of more than 70% of β-carotene compared to the wild-type sibling lines, with no change in lutein, α-carotene and zeaxanthin content. This study sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat and provides new genotypes that represent a good genetic resource for future breeding programs focused on the provitamin A biofortification through non-transgenic approaches.it
dc.format.mediumELETTRONICOit
dc.language.isoengit
dc.titleEnrichment of provitamin A content in durum wheat grain by suppressing the beta-carotene hydroxylase 1 with a TILLING approachit
dc.typearticle*
dc.identifier.doihttps://doi.org/10.1007/s00122-021-03944-6it
dc.identifier.scopus2-s2.0-85114182105it
dc.relation.journalTHEORETICAL AND APPLIED GENETICSit
dc.relation.numberofpages12it
dc.subject.scientificsectorAGR/07it
dc.subject.keywordsdurum wheat, vitamin A, beta-carotene, TILLINGit
dc.subject.ercsectorLS9_5it
dc.description.numberofauthors8it
dc.description.internationalit
dc.contributor.countryITAit
dc.type.refereeREF_1it
dc.type.miur262*
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
crisitem.journal.journalissn1432-2242-
crisitem.journal.anceE213242-
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