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Title: First report of bud rot caused by Cryptosporiopsis tarraconensis on Corylus avellana in Italy
Authors: Tagliavento, Vincenzo
de Santis, Federico
Ciarroni, Serena
Balestra, Giorgio Mariano 
Cristofori, Valerio 
Pradolesi, Gianfranco
Issue Date: 2021
During seasons 2018 and 2019, an intense monitoring activity was carried out on ten fields of common hazel (Corylus avellana L.), for a total of approximately 25.000 plants, located in the growing areas of Tuscia, the northern territory of Lazio region (Italy). Desiccation of minor branches and its associated leaves, and, occasionally, dry rot of buds, leading to their abortion, were observed on about 30% of mature common hazel trees during the field surveys, in particular on cv. Nocchione; the symptoms were less evident on cv. Tonda
Gentile Romana and cv. Tonda Giffoni. Fungal isolation from symptomatic branches and buds was performed: small portions of symptomatic samples were surface disinfected in 2% sodium hypoclorite for 60 s, rinsed in sterile distilled water and transferred on potato dextrose agar (PDA). After 2 weeks
of incubation at 25 °C ± 2 °C, a whitish to light brown mycelium, more raised in the center, was observed; after about 20 days of incubation, dark brown globular bodies also developed. Under the light microscope, acervuli (150–165 X 90–105 μm) appeared to contain aseptate, hyaline and elongate to cylindrical small conidia (7.5–16 X 4–6 μm), characterized by an evident basal scar. All the macroscopic and microscopic
morphological traits so far described suggested to be in the presence of the fungus Cryptosporiopsis tarraconensis Gené& Guarro (Gené et al. 1990). Genomic DNA from pure mycelium of a reference isolate of the pathogen was extracted by using a commercial kit and it was used as template to amplify
and sequence the Internal Transcribed Spacer (ITS) region by ITS1 and ITS4 primers. The obtained sequence
(GenBank accession No. MT012300) showed 99% query and 100% identity to C. tarraconensis ITS region sequence deposited into NCBI database (EU707431.1). Pathogenicitytest was carried out depositing a PDA disk, obtained from apure culture of mycelium of the reference isolate numbered2.7 preserved in the laboratory collection, on scalpel-wounded young branches of 2-years-old hazelnut cv. Nocchione: within20 days, desiccation symptoms pretty much similar to those observed in the field appeared on all inoculated plants, but not on the control plants inoculated with a sterile PDA disk. C. tarraconensis was re-isolated from all the symptomatic plants according to the protocol described above. This fungal pathogen was firstly recognized as agent of bud rot or “borró sec” on hazelnut in Spain (Gené et al. 1990). More recently, it has been also indicated as responsible for foliar brown spots on hazelnut (Roohvarzi et al. 2013). To our knowledge, this is
the first time that the presence of C. tarraconensis is reported in Italy.
ISSN: 2239-7264
DOI: 10.1007/s42161-020-00680-6
Rights: Attribution-NonCommercial 3.0 United States
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