Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/43295
DC Field | Value | Language |
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dc.contributor.author | Miccoli, Andrea | it |
dc.contributor.author | Guerra, Laura | it |
dc.contributor.author | Pianese, Valeria | it |
dc.contributor.author | Saraceni, Paolo Roberto | it |
dc.contributor.author | Buonocore, Francesco | it |
dc.contributor.author | Taddei, Anna Rita | it |
dc.contributor.author | Couto, Ana | it |
dc.contributor.author | De Wolf, Tania | it |
dc.contributor.author | Fausto, Anna Maria | it |
dc.contributor.author | Scapigliati, Giuseppe | it |
dc.contributor.author | Picchietti, Simona | it |
dc.date.accessioned | 2021-04-08T10:18:24Z | - |
dc.date.available | 2021-04-08T10:18:24Z | - |
dc.date.issued | 2021 | it |
dc.identifier.issn | 1422-0067 | it |
dc.identifier.uri | http://hdl.handle.net/2067/43295 | - |
dc.description.abstract | In jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide chains, which display an Ig domain‐type sequence that is somatically rearranged. γδ T cells have been less extensively characterized than αβ and teleost fish, in particular, suffer from a severe scarcity of data. In this paper, we worked on the well‐known model, the European sea bass Dicentrarchus labrax, to broaden the understanding of teleost γδ‐T cells. The T cell receptor chain (TR) γ transcript was expressed at a later developmental stage than TRβ, suggesting a layered appearance of fish immune cells, and the thymus displayed statistically‐significant higher mRNA levels than any other organ or lymphoid tissue investigated. The polyclonal antibody developed against the TRγ allowed the localization of TRγ‐expressing cells in lymphoid organs along the ontogeny. Cell positivity was investigated through flow cytometry and the highest percentage was found in peripheral blood leukocytes, followed by thymus, gut, gills, spleen and head kidney. Numerous TRγ‐expressing cells were localized in the gut mucosa, and the immunogold labelling revealed ultrastructural features that are typical of T cells. At last, microalgae‐based diet formulations significantly modulated the abundance of TRγ+ cells in the posterior intestine, hinting at a putative involvement in nutritional immunity. From a comparative immunological perspective, our results contribute to the comprehension of the diversity and functionalities of γδ T cells during the development of a commercially relevant marine teleost model. | it |
dc.format.medium | ELETTRONICO | it |
dc.language.iso | eng | it |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.title | Molecular, Cellular and Functional Analysis of TRγ Chain along the European Sea Bass Dicentrarchus labrax Development | it |
dc.type | article | * |
dc.identifier.doi | 10.3390/ijms22073376 | it |
dc.identifier.scopus | 2-s2.0-85103013404 | it |
dc.relation.journal | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | it |
dc.relation.firstpage | 3376 | it |
dc.relation.numberofpages | 21 | it |
dc.relation.volume | 22 | it |
dc.relation.issue | 7 | it |
dc.subject.scientificsector | BIO/10 | it |
dc.subject.keywords | T cell receptor gamma chain | it |
dc.subject.keywords | immune system | it |
dc.subject.keywords | ontogeny | it |
dc.subject.ercsector | LS2 | it |
dc.description.numberofauthors | 11 | it |
dc.description.international | sì | it |
dc.contributor.country | ITA | it |
dc.type.referee | REF_1 | it |
dc.type.miur | 262 | * |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | restricted | - |
item.openairetype | article | - |
crisitem.journal.journalissn | 1422-0067 | - |
crisitem.journal.ance | E184525 | - |
Appears in Collections: | A1. Articolo in rivista |
Files in This Item:
File | Description | Size | Format | Existing users please |
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TRgammaijms-22-03376.pdf | TRgamma paper | 2.61 MB | Adobe PDF | Request a copy |
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