Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43295
DC FieldValueLanguage
dc.contributor.authorMiccoli, Andreait
dc.contributor.authorGuerra, Laurait
dc.contributor.authorPianese, Valeriait
dc.contributor.authorSaraceni, Paolo Robertoit
dc.contributor.authorBuonocore, Francescoit
dc.contributor.authorTaddei, Anna Ritait
dc.contributor.authorCouto, Anait
dc.contributor.authorDe Wolf, Taniait
dc.contributor.authorFausto, Anna Mariait
dc.contributor.authorScapigliati, Giuseppeit
dc.contributor.authorPicchietti, Simonait
dc.date.accessioned2021-04-08T10:18:24Z-
dc.date.available2021-04-08T10:18:24Z-
dc.date.issued2021it
dc.identifier.issn1422-0067it
dc.identifier.urihttp://hdl.handle.net/2067/43295-
dc.description.abstractIn jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide chains, which display an Ig domain‐type sequence that is somatically rearranged. γδ T cells have been less extensively characterized than αβ and teleost fish, in particular, suffer from a severe scarcity of data. In this paper, we worked on the well‐known model, the European sea bass Dicentrarchus labrax, to broaden the understanding of teleost γδ‐T cells. The T cell receptor chain (TR) γ transcript was expressed at a later developmental stage than TRβ, suggesting a layered appearance of fish immune cells, and the thymus displayed statistically‐significant higher mRNA levels than any other organ or lymphoid tissue investigated. The polyclonal antibody developed against the TRγ allowed the localization of TRγ‐expressing cells in lymphoid organs along the ontogeny. Cell positivity was investigated through flow cytometry and the highest percentage was found in peripheral blood leukocytes, followed by thymus, gut, gills, spleen and head kidney. Numerous TRγ‐expressing cells were localized in the gut mucosa, and the immunogold labelling revealed ultrastructural features that are typical of T cells. At last, microalgae‐based diet formulations significantly modulated the abundance of TRγ+ cells in the posterior intestine, hinting at a putative involvement in nutritional immunity. From a comparative immunological perspective, our results contribute to the comprehension of the diversity and functionalities of γδ T cells during the development of a commercially relevant marine teleost model.it
dc.format.mediumELETTRONICOit
dc.language.isoengit
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.titleMolecular, Cellular and Functional Analysis of TRγ Chain along the European Sea Bass Dicentrarchus labrax Developmentit
dc.typearticle*
dc.identifier.doi10.3390/ijms22073376it
dc.identifier.scopus2-s2.0-85103013404it
dc.relation.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCESit
dc.relation.firstpage3376it
dc.relation.numberofpages21it
dc.relation.volume22it
dc.relation.issue7it
dc.subject.scientificsectorBIO/10it
dc.subject.keywordsT cell receptor gamma chainit
dc.subject.keywordsimmune systemit
dc.subject.keywordsontogenyit
dc.subject.ercsectorLS2it
dc.description.numberofauthors11it
dc.description.internationalit
dc.contributor.countryITAit
dc.type.refereeREF_1it
dc.type.miur262*
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairetypearticle-
crisitem.journal.journalissn1422-0067-
crisitem.journal.anceE184525-
Appears in Collections:A1. Articolo in rivista
Files in This Item:
File Description SizeFormat Existing users please
TRgammaijms-22-03376.pdfTRgamma paper2.61 MBAdobe PDF    Request a copy
Show simple item record

SCOPUSTM   
Citations 20

6
Last Week
0
Last month
0
checked on Oct 27, 2024

Page view(s)

147
Last Week
1
Last month
2
checked on Nov 2, 2024

Download(s)

4
checked on Nov 2, 2024

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons