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Title: Molecular, Cellular and Functional Analysis of TRγ Chain along the European Sea Bass Dicentrarchus labrax Development
Authors: Miccoli, Andrea 
Guerra, Laura
Pianese, Valeria
Saraceni, Paolo Roberto
Buonocore, Francesco 
Taddei, Anna Rita
Couto, Ana
De Wolf, Tania
Fausto, Anna Maria 
Scapigliati, Giuseppe 
Picchietti, Simona 
Issue Date: 2021
In jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages
were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide
chains, which display an Ig domain‐type sequence that is somatically rearranged. γδ T cells have
been less extensively characterized than αβ and teleost fish, in particular, suffer from a severe
scarcity of data. In this paper, we worked on the well‐known model, the European sea bass
Dicentrarchus labrax, to broaden the understanding of teleost γδ‐T cells. The T cell receptor chain
(TR) γ transcript was expressed at a later developmental stage than TRβ, suggesting a layered
appearance of fish immune cells, and the thymus displayed statistically‐significant higher mRNA
levels than any other organ or lymphoid tissue investigated. The polyclonal antibody developed
against the TRγ allowed the localization of TRγ‐expressing cells in lymphoid organs along the
ontogeny. Cell positivity was investigated through flow cytometry and the highest percentage was
found in peripheral blood leukocytes, followed by thymus, gut, gills, spleen and head kidney.
Numerous TRγ‐expressing cells were localized in the gut mucosa, and the immunogold labelling
revealed ultrastructural features that are typical of T cells. At last, microalgae‐based diet
formulations significantly modulated the abundance of TRγ+ cells in the posterior intestine, hinting
at a putative involvement in nutritional immunity. From a comparative immunological perspective,
our results contribute to the comprehension of the diversity and functionalities of γδ T cells during
the development of a commercially relevant marine teleost model.
ISSN: 1422-0067
DOI: 10.3390/ijms22073376
Rights: Attribution-NonCommercial-NoDerivs 3.0 United States
Appears in Collections:A1. Articolo in rivista

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