Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43290
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dc.contributor.authorKlionsky, Daniel J.it
dc.contributor.authorAbdel-Aziz, Amal Kamalit
dc.contributor.authorAbdelfatah, Sarait
dc.contributor.authorAbdellatif, Mahmoudit
dc.contributor.authorAbdoli, Asgharit
dc.contributor.authorAbel, Steffenit
dc.contributor.authorAbeliovich, Hagaiit
dc.contributor.authorAbildgaard, Marie H.it
dc.contributor.authorAbudu, Yakubu Princelyit
dc.contributor.authorAcevedo-Arozena, Abrahamit
dc.contributor.authorAdamopoulos, Iannis E.it
dc.contributor.authorAdeli, Khosrowit
dc.contributor.authorAdolph, Timon E.it
dc.contributor.authorAdornetto, Annagraziait
dc.contributor.authorAflaki, Elmait
dc.contributor.authorAgam, Galilait
dc.contributor.authorAgarwal, Anupamit
dc.contributor.authorAggarwal, Bharat B.it
dc.contributor.authorAgnello, Mariait
dc.contributor.authorAgostinis, Patriziait
dc.contributor.authorAgrewala, Javed N.it
dc.contributor.authorAgrotis, Alexanderit
dc.contributor.authorAguilar, Patricia V.it
dc.contributor.authorAhmad, S. Tariqit
dc.contributor.authorAhmed, Zubair M.it
dc.contributor.authorAhumada-Castro, Ulisesit
dc.contributor.authorAits, Sonjait
dc.contributor.authorAizawa, Shuit
dc.contributor.authorAkkoc, Yunusit
dc.contributor.authorAkoumianaki, Toniait
dc.contributor.authorAkpinar, Hafize Aysinit
dc.contributor.authorAl-Abd, Ahmed M.it
dc.contributor.authorAl-Akra, Linait
dc.contributor.authorAl-Gharaibeh, Abeerit
dc.contributor.authorAlaoui-Jamali, Moulay A.it
dc.contributor.authorAlberti, Simonit
dc.contributor.authorAlcocer-Gómez, Elísabetit
dc.contributor.authorAlessandri, Cristianoit
dc.contributor.authorAli, Muhammadit
dc.contributor.authorAlim Al-Bari, M. Abdulit
dc.contributor.authorAliwaini, Saebit
dc.contributor.authorAlizadeh, Javadit
dc.contributor.authorAlmacellas, Eugèniait
dc.contributor.authorAlmasan, Alexandruit
dc.contributor.authorAlonso, Aliciait
dc.contributor.authorAlonso, Guillermo D.it
dc.contributor.authorAltan-Bonnet, Nihalit
dc.contributor.authorAltieri, Dario C.it
dc.contributor.authorÁlvarez, Élida M.C.it
dc.contributor.authorAlves, Sarait
dc.contributor.authorAlves da Costa, Cristineit
dc.contributor.authorAlzaharna, Mazen M.it
dc.contributor.authorAmadio, Marialaurait
dc.contributor.authorAmantini, Consueloit
dc.contributor.authorAmaral, Cristinait
dc.contributor.authorAmbrosio, Susannait
dc.contributor.authorAmer, Amal O.it
dc.contributor.authorAmmanathan, Veenait
dc.contributor.authorAn, Zhenyiit
dc.contributor.authorAndersen, Stig U.it
dc.contributor.authorAndrabi, Shaida A.it
dc.contributor.authorAndrade-Silva, Magaiverit
dc.contributor.authorAndres, Allen M.it
dc.contributor.authorAngelini, Sabrinait
dc.contributor.authorAnn, Davidit
dc.contributor.authorAnozie, Uche C.it
dc.contributor.authorAnsari, Mohammad Y.it
dc.contributor.authorAntas, Pedroit
dc.contributor.authorAntebi, Adamit
dc.contributor.authorAntón, Zuriñeit
dc.contributor.authorAnwar, Tahirait
dc.contributor.authorApetoh, Lionelit
dc.contributor.authorApostolova, Nadezdait
dc.contributor.authorAraki, Toshiyukiit
dc.contributor.authorAraki, Yasuhiroit
dc.contributor.authorArasaki, Koheiit
dc.contributor.authorAraújo, Wagner L.it
dc.contributor.authorAraya, Junit
dc.contributor.authorArden, Catherineit
dc.contributor.authorArévalo, Maria Angelesit
dc.contributor.authorArguelles, Sandroit
dc.contributor.authorArias, Esperanzait
dc.contributor.authorArikkath, Jyothiit
dc.contributor.authorArimoto, Hirokazuit
dc.contributor.authorAriosa, Aileen R.it
dc.contributor.authorArmstrong-James, Dariusit
dc.contributor.authorArnauné-Pelloquin, Laetitiait
dc.contributor.authorAroca, Angelesit
dc.contributor.authorArroyo, Daniela S.it
dc.contributor.authorArsov, Ivicait
dc.contributor.authorArtero, Rubénit
dc.contributor.authorAsaro, Dalia Maria Luciait
dc.contributor.authorAschner, Michaelit
dc.contributor.authorAshrafizadeh, Miladit
dc.contributor.authorAshur-Fabian, Osnatit
dc.contributor.authorAtanasov, Atanas G.it
dc.contributor.authorAu, Alicia K.it
dc.contributor.authorAuberger, Patrickit
dc.contributor.authorAuner, Holger W.it
dc.contributor.authorAurelian, Laureit
dc.contributor.authorVelotti, Francesca Romanait
dc.date.accessioned2021-04-06T15:54:16Z-
dc.date.available2021-04-06T15:54:16Z-
dc.date.issued2021it
dc.identifier.issn1554-8627it
dc.identifier.urihttp://hdl.handle.net/2067/43290-
dc.description.abstractIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.it
dc.language.isoengit
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.titleGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition)it
dc.typearticle*
dc.identifier.doi10.1080/15548627.2020.1797280it
dc.identifier.pmid33634751it
dc.identifier.scopus2-s2.0-85102619204it
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85102619204it
dc.relation.journalAUTOPHAGYit
dc.relation.firstpage1-382it
dc.relation.lastpage382it
dc.relation.volume17it
dc.relation.issue1it
dc.description.internationalit
dc.type.miur262*
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn1554-8627-
crisitem.journal.anceE188924-
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