Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43175
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dc.contributor.authorBuonocore, Francescoit
dc.contributor.authorGerdol, Marcoit
dc.contributor.authorPallavicini, Albertoit
dc.contributor.authorStocchi, Valentinait
dc.contributor.authorRandelli, Elisait
dc.contributor.authorBelardinelli, Maria Cristinait
dc.contributor.authorMiccoli, Andreait
dc.contributor.authorSaraceni, Paolo Robertoit
dc.contributor.authorSecombes, Christopher Jit
dc.contributor.authorScapigliati, Giuseppeit
dc.contributor.authorWang, Tiehuiit
dc.date.accessioned2021-03-16T14:23:50Z-
dc.date.available2021-03-16T14:23:50Z-
dc.date.issued2020it
dc.identifier.issn1043-4666it
dc.identifier.urihttp://hdl.handle.net/2067/43175-
dc.description.abstractIn mammals, interleukin (IL)-2, initially known as a T-cell grow factor, is an immunomodulatory cytokine involved in the proliferation of T cells upon antigen activation. In bony fish, some IL-2 orthologs have been identified, but, recently, an additional IL-2like (IL-2L) gene has been found. In this paper, we report the presence of these two divergent IL-2 isoforms in sea bass (Dicentrarchus labrax L.). Genomic analyses revealed that they originated from a gene duplication event, as happened in most percomorphs. These two IL-2 paralogs show differences in the amino acid sequence and in the exon 4 size, and these features could be an indication that they bind preferentially to different specific IL-2 receptors. Sea bass IL-2 paralogs are highly expressed in gut and spleen, which are tissues and organs involved in fish T cell immune functions, and the two cytokines could be up-regulated by both PHA stimulation and vaccination with a bacterial vaccine, with IL-2L being more inducible. To investigate the functional activities of sea bass IL-2 and IL-2L we produced the corresponding recombinant molecules in E. coli and used them to in vitro stimulate HK and spleen leukocytes. IL-2L is able to up-regulate the expression of markers related to different T cell subsets (Th1, Th2 and Th17) and to Treg cells in HK, whereas it has little effect in spleen. IL-2 is not active on these markers in HK, but shows an effect on Th1 markers in spleen. Finally, the stimulation with recombinant IL-2 and IL-2L is also able to induce in vitro proliferation of HK- and spleen-derived leukocytes. In conclusion, we have demonstrated that sea bass possess two IL-2 paralogs that likely have an important role in regulating T cell development in this species and that show distinct bioactivities.it
dc.format.mediumELETTRONICOit
dc.language.isoengit
dc.titleIdentification, molecular characterization and functional analysis of interleukin (IL)-2 and IL-2like (IL-2L) cytokines in sea bass (Dicentrarchus labrax L.)it
dc.typearticle*
dc.identifier.doi10.1016/j.cyto.2019.154898it
dc.identifier.pmid31706201it
dc.identifier.scopus2-s2.0-85074537285it
dc.identifier.isi000509612400026it
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85074537285it
dc.relation.journalCYTOKINEit
dc.relation.firstpage154898it
dc.relation.volume126it
dc.description.numberofauthors11it
dc.description.internationalit
dc.contributor.countryITAit
dc.contributor.countryGBRit
dc.type.miur262*
item.fulltextWith Fulltext-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.journal.journalissn1043-4666-
crisitem.journal.anceE047131-
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