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http://hdl.handle.net/2067/43035
Campo DC | Valore | Lingua |
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dc.contributor.author | Sestili, Francesco | it |
dc.contributor.author | Garcia-Molina, Maria Dolores | it |
dc.contributor.author | Gambacorta, Gianluca | it |
dc.contributor.author | Beleggia, Romina | it |
dc.contributor.author | Botticella, Ermelinda | it |
dc.contributor.author | De Vita, Pasquale | it |
dc.contributor.author | Savatin, Daniel Valentin | it |
dc.contributor.author | Masci, Stefania | it |
dc.contributor.author | Lafiandra, Domenico | it |
dc.date.accessioned | 2021-02-25T17:37:15Z | - |
dc.date.available | 2021-02-25T17:37:15Z | - |
dc.date.issued | 2019 | it |
dc.identifier.issn | 1661-6596 | it |
dc.identifier.uri | http://hdl.handle.net/2067/43035 | - |
dc.description.abstract | Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a "block strategy" perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat. | it |
dc.language.iso | eng | it |
dc.rights | CC0 1.0 Universal | * |
dc.rights.uri | http://creativecommons.org/publicdomain/zero/1.0/ | * |
dc.title | Provitamin A Biofortification of Durum Wheat through a TILLING Approach | it |
dc.type | article | * |
dc.identifier.doi | 10.3390/ijms20225703 | it |
dc.identifier.pmid | 31739436 | it |
dc.identifier.scopus | 2-s2.0-85075114701 | it |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85075114701 | it |
dc.relation.journal | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | it |
dc.relation.firstpage | 5703 | it |
dc.relation.volume | 20 | it |
dc.relation.issue | 22 | it |
dc.description.numberofauthors | 9 | it |
dc.description.international | no | it |
dc.contributor.country | ITA | it |
dc.type.referee | REF_1 | it |
dc.type.miur | 262 | * |
item.fulltext | With Fulltext | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | restricted | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.journal.journalissn | 1661-6596 | - |
crisitem.journal.ance | E217024 | - |
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