Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43035
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dc.contributor.authorSestili, Francescoit
dc.contributor.authorGarcia-Molina, Maria Doloresit
dc.contributor.authorGambacorta, Gianlucait
dc.contributor.authorBeleggia, Rominait
dc.contributor.authorBotticella, Ermelindait
dc.contributor.authorDe Vita, Pasqualeit
dc.contributor.authorSavatin, Daniel Valentinit
dc.contributor.authorMasci, Stefaniait
dc.contributor.authorLafiandra, Domenicoit
dc.date.accessioned2021-02-25T17:37:15Z-
dc.date.available2021-02-25T17:37:15Z-
dc.date.issued2019it
dc.identifier.issn1661-6596it
dc.identifier.urihttp://hdl.handle.net/2067/43035-
dc.description.abstractMacro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a "block strategy" perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat.it
dc.language.isoengit
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.titleProvitamin A Biofortification of Durum Wheat through a TILLING Approachit
dc.typearticle*
dc.identifier.doi10.3390/ijms20225703it
dc.identifier.pmid31739436it
dc.identifier.scopus2-s2.0-85075114701it
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85075114701it
dc.relation.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCESit
dc.relation.firstpage5703it
dc.relation.volume20it
dc.relation.issue22it
dc.description.numberofauthors9it
dc.description.internationalnoit
dc.contributor.countryITAit
dc.type.refereeREF_1it
dc.type.miur262*
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.languageiso639-1en-
crisitem.journal.journalissn1661-6596-
crisitem.journal.anceE217024-
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