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Title: Provitamin A Biofortification of Durum Wheat through a TILLING Approach
Authors: Sestili, Francesco 
Garcia-Molina, Maria Dolores
Gambacorta, Gianluca
Beleggia, Romina
Botticella, Ermelinda
De Vita, Pasquale
Savatin, Daniel Valentin 
Masci, Stefania
Lafiandra, Domenico 
Issue Date: 2019
Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a "block strategy" perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat.
ISSN: 1661-6596
DOI: 10.3390/ijms20225703
Rights: CC0 1.0 Universal
Appears in Collections:A1. Articolo in rivista

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