Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43032
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dc.contributor.authorKalunke, Raviraj Mit
dc.contributor.authorTundo, Silvioit
dc.contributor.authorSestili, Francescoit
dc.contributor.authorCamerlengo, Francescoit
dc.contributor.authorLafiandra, Domenicoit
dc.contributor.authorLupi, Robertait
dc.contributor.authorLarré, Coletteit
dc.contributor.authorDenery-Papini, Sandrait
dc.contributor.authorIslam, Shahidulit
dc.contributor.authorMa, Wujunit
dc.contributor.authorD'Amico, Stefanoit
dc.contributor.authorMasci, Stefaniait
dc.date.accessioned2021-02-25T17:23:00Z-
dc.date.available2021-02-25T17:23:00Z-
dc.date.issued2020it
dc.identifier.issn1661-6596it
dc.identifier.urihttp://hdl.handle.net/2067/43032-
dc.description.abstractAlthough wheat is used worldwide as a staple food, it can give rise to adverse reactions, for which the triggering factors have not been identified yet. These reactions can be caused mainly by kernel proteins, both gluten and non-gluten proteins. Among these latter proteins, α-amylase/trypsin inhibitors (ATI) are involved in baker's asthma and realistically in Non Celiac Wheat Sensitivity (NCWS). In this paper, we report characterization of three transgenic lines obtained from the bread wheat cultivar Bobwhite silenced by RNAi in the three ATI genes CM3, CM16 and 0.28. We have obtained transgenic lines showing an effective decrease in the activity of target genes that, although showing a higher trypsin inhibition as a pleiotropic effect, generate a lower reaction when tested with sera of patients allergic to wheat, accounting for the important role of the three target proteins in wheat allergies. Finally, these lines show unintended differences in high molecular weight glutenin subunits (HMW-GS) accumulation, involved in technological performances, but do not show differences in terms of yield. The development of new genotypes accumulating a lower amount of proteins potentially or effectively involved in allergies to wheat and NCWS, not only offers the possibility to use them as a basis for the production of varieties with a lower impact on adverse reaction, but also to test if these proteins are actually implicated in those pathologies for which the triggering factor has not been established yet.it
dc.format.mediumELETTRONICOit
dc.language.isoengit
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.titleReduction of Allergenic Potential in Bread Wheat RNAi Transgenic Lines Silenced for CM3, CM16 and 0.28ATI Genesit
dc.typearticle*
dc.identifier.doi10.3390/ijms21165817it
dc.identifier.pmid32823634it
dc.identifier.scopus2-s2.0-85089590859it
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85089590859it
dc.relation.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCESit
dc.relation.firstpage1it
dc.relation.lastpage21it
dc.relation.volume21it
dc.relation.issue16it
dc.description.internationalit
dc.contributor.countryITAit
dc.type.refereeREF_1it
dc.type.miur262*
item.fulltextWith Fulltext-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.journal.journalissn1661-6596-
crisitem.journal.anceE217024-
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