Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/43032
Title: Reduction of Allergenic Potential in Bread Wheat RNAi Transgenic Lines Silenced for CM3, CM16 and 0.28ATI Genes
Authors: Kalunke, Raviraj M
Tundo, Silvio
Sestili, Francesco 
Camerlengo, Francesco
Lafiandra, Domenico 
Lupi, Roberta
Larré, Colette
Denery-Papini, Sandra
Islam, Shahidul
Ma, Wujun
D'Amico, Stefano
Masci, Stefania 
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date: 2020
Abstract: 
Although wheat is used worldwide as a staple food, it can give rise to adverse reactions, for which the triggering factors have not been identified yet. These reactions can be caused mainly by kernel proteins, both gluten and non-gluten proteins. Among these latter proteins, α-amylase/trypsin inhibitors (ATI) are involved in baker's asthma and realistically in Non Celiac Wheat Sensitivity (NCWS). In this paper, we report characterization of three transgenic lines obtained from the bread wheat cultivar Bobwhite silenced by RNAi in the three ATI genes CM3, CM16 and 0.28. We have obtained transgenic lines showing an effective decrease in the activity of target genes that, although showing a higher trypsin inhibition as a pleiotropic effect, generate a lower reaction when tested with sera of patients allergic to wheat, accounting for the important role of the three target proteins in wheat allergies. Finally, these lines show unintended differences in high molecular weight glutenin subunits (HMW-GS) accumulation, involved in technological performances, but do not show differences in terms of yield. The development of new genotypes accumulating a lower amount of proteins potentially or effectively involved in allergies to wheat and NCWS, not only offers the possibility to use them as a basis for the production of varieties with a lower impact on adverse reaction, but also to test if these proteins are actually implicated in those pathologies for which the triggering factor has not been established yet.
URI: http://hdl.handle.net/2067/43032
ISSN: 1661-6596
DOI: 10.3390/ijms21165817
Rights: CC0 1.0 Universal
Appears in Collections:A1. Articolo in rivista

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