Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/42948
DC FieldValueLanguage
dc.contributor.authorCeci, Marcelloit
dc.contributor.authorCarlantoni, Claudiait
dc.contributor.authorMissinato, Maria Azzurrait
dc.contributor.authorBonvissuto, Davideit
dc.contributor.authorDi Giacomo, Brunait
dc.contributor.authorContu, Riccardoit
dc.contributor.authorRomano, Niclait
dc.date.accessioned2021-02-22T12:25:45Z-
dc.date.available2021-02-22T12:25:45Z-
dc.date.issued2018it
dc.identifier.issn2058-7716it
dc.identifier.urihttp://hdl.handle.net/2067/42948-
dc.description.abstractZebrafish could be an interesting translational model to understand and improve the post-infarction trial and possible regeneration in humans. The adult zebrafish is able to regenerate efficiently after resecting nearly 20% of the ventricular apex. This process requires the concert activation of the epicardium and endocardium, as well as trans-differentiation of pre-existing cardiomyocytes that together replace the lost tissue. The molecular mechanisms involved in this activation process are not completely clarified. In this work, in order to investigate if the downregulation of these miRNAs (miRs) are linked with the activation of epicardium, the expressions of miR-133a, b and miR-1 during regeneration were analysed. qPCR analyses in whole-heart, or from distinct dissected epicardial cells comparing to regenerative clot (containing cardiomyocytes, fibroblasts and endocardial cells) by a laser-micro-dissector, have indicated that already at 24 h there is a downregulation of miRs: (1) miR-133a and miR-1 in the epicardium and (2) miR-133b and miR-1 in the regenerative clot. All the miRs remain downregulated until 7 days post-surgery. With the aim to visualize the activations of heart component in combination with miRs, we developed immunohistochemistry using antibodies directed against common markers in mammals as well as zebrafish: Wilms tumour 1 (WT1), a marker of epicardium; heat-shock protein 70 (HSP70), a chaperon activated during regeneration; and the Cardiac Troponin T (cTnT), a marker of differentiated cardiomyocytes. All these markers are directly or indirectly linked to the investigated miRs. WT1 and HSP70 strongly marked the regeneration site just at 2-3 days postventricular resection. In coherence, cTnT intensively marked the regenerative portion from 7 days onwards. miRs-1 and -133 (a,b) have been strongly involved in the activation of epicardium and regenerative clot during the regeneration process in zebrafish. This study can be a useful translational model to understand the early epicardial activation in which miRs-133a and miR-1 seem to play a central role as observed in the human heart.it
dc.format.mediumELETTRONICOit
dc.language.isoengit
dc.rightsAttribution 4.0 International-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.titleMicro RNAs are involved in activation of epicardium during zebrafish heart regenerationit
dc.typearticle*
dc.identifier.doi10.1038/s41420-018-0041-xit
dc.identifier.pmid29560280it
dc.identifier.scopus2-s2.0-85047849996it
dc.identifier.urlhttps://dspace.unitus.it/handle/2067/37299it
dc.identifier.urlwww.nature.com/articles/s41420-018-0041-xit
dc.relation.journalCELL DEATH DISCOVERYit
dc.relation.firstpage1it
dc.relation.lastpage13it
dc.relation.numberofpages13it
dc.relation.article41it
dc.relation.volume4it
dc.relation.issue1it
dc.subject.scientificsector05-B2/BIO 06it
dc.subject.keywordszebrafish, Cardiac regeneration, microRNAsit
dc.subject.ercsectorLSit
dc.description.numberofauthors6it
dc.description.internationalnoit
dc.type.refereeREF_1it
dc.type.miur262*
item.fulltextWith Fulltext-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.journal.journalissn2058-7716-
crisitem.journal.anceE232923-
Appears in Collections:A1. Articolo in rivista
Files in This Item:
File Description SizeFormat Existing users please
CD Discovery 2018.PDF2.72 MBAdobe PDF    Request a copy
Show simple item record

SCOPUSTM   
Citations 10

10
Last Week
0
Last month
1
checked on Mar 22, 2024

Page view(s)

121
Last Week
0
Last month
3
checked on Mar 27, 2024

Download(s)

3
checked on Mar 27, 2024

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons