Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/42939
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dc.contributor.authorRomano, Niclait
dc.contributor.authorCeci, Marcelloit
dc.date.accessioned2021-02-22T09:47:23Z-
dc.date.available2021-02-22T09:47:23Z-
dc.date.issued2020it
dc.identifier.issn1879-260Xit
dc.identifier.urihttp://hdl.handle.net/2067/42939-
dc.description.abstractRecent studies have correlated dysregulated miRNA expression with diseased hearts. With the aim of developing an easily manipulated experimental model, phenylephrine (PE) was added to cultured zebrafish hearts to study the expression of miR1 and miR133a by qRT-PCR. Both miRs were downregulated, with greater downregulation leading to higher hypertrophy. The involvement of this miRs was confirmed by the in-vivo inoculation of complementary sequences (AmiR1 and AmiR133a). HSP70 (involved in transporting proteins and in anti-apoptosis processes) was increased in both treatments. Hyperplasia was observed in the epicardium based on WT1 expression (embryonic epicardial cell marker) in both the PE treatment and AmiR133a treatment. The treatment with AmiR1 showed only cardiomyocyte hypertrophy. This ex-vivo model revealed that miR1 and miR133a play a key role in activating early processes leading to myocardium hypertrophy and epicardium hyperplasia and confirmed the expected similarities with hypertrophic disease that occurs in humans.it
dc.format.mediumSTAMPAit
dc.language.isoengit
dc.titleAre microRNAs responsible for cardiac hypertrophy in fish and mammals? What we can learn in the activation process in a zebrafish ex vivo modelit
dc.typearticle*
dc.identifier.doi10.1016/j.bbadis.2020.165896it
dc.identifier.pmid32681863it
dc.identifier.scopus2-s2.0-85088868573it
dc.identifier.urlhttps://www.sciencedirect.com/science/article/abs/pii/S0925443920302441?via%3Dihubit
dc.relation.journalBIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR BASIS OF DISEASEit
dc.relation.firstpage1it
dc.relation.lastpage11it
dc.relation.numberofpages11it
dc.relation.article165896it
dc.relation.volume1866it
dc.relation.issue11it
dc.subject.scientificsectorBIO-06it
dc.subject.keywordscardiac hypertrophyit
dc.subject.keywordsHeat-Shock Proteinsit
dc.subject.keywordsMicroRNAsit
dc.subject.keywordsPhenylephrineit
dc.subject.keywordsex vivo hypertrophy modelit
dc.subject.keywordszebrafishit
dc.subject.ercsectorLS3_12it
dc.description.numberofauthors2it
dc.description.internationalnoit
dc.description.noteprimo modello ex-vivo di ipertrofia cardiaca in un pesceit
dc.contributor.countryITAit
dc.type.refereeREF_1it
dc.type.miur262it
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypearticle-
crisitem.journal.journalissn1879-260X-
crisitem.journal.anceE252162-
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