Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/2067/42809
Titolo: | Untargeted Metabolomics of Plant Leaf Tissues | Autori: | Gevi, Federica Fanelli, Giuseppina Zolla, Lello Rinalducci, Sara |
Data pubblicazione: | 2019 | Abstract: | Untargeted metabolomics is a useful approach for the simultaneous analysis of a vast array of compounds from a single extract. Metabolomic profiling is the relative multi-parallel quantification of a mixture of low molecular weight compounds, or classes of compounds, and it is most often performed by using ultra performance liquid chromatography (UPLC) coupled with mass spectrometry (MS). Being an extension of the classical targeted methods, this approach allows a broader view of the main biochemical events within a particular sample. This chapter exemplifies and provides experimental details on the basic steps to perform a non-targeted metabolomic analysis on plant leaf tissues: sample collection and homogenization, extraction of metabolites, raw data acquisition, and processing into formats for data mining and informatics. In particular, the approach was applied to two spring wheat varieties with different level of drought tolerance (Kavir, drought-resistant; Bahar, drought-sensitive) developed by the CIMMYT (International Center for the Improvement of Corn and Wheat). |
URI: | http://hdl.handle.net/2067/42809 | ISBN: | 978-1-4939-9235-5 | DOI: | 10.1007/978-1-4939-9236-2_12 |
È visualizzato nelle collezioni: | B1. Contributo in volume (Capitolo o Saggio) |
File in questo documento:
File | Descrizione | Dimensioni | Formato | Existing users please |
---|---|---|---|---|
Plant Metabolomics Protocol_accepted version.pdf | AAM | 574.54 kB | Adobe PDF | Richiedi una copia |
SCOPUSTM
Citations
20
3
Last Week
0
0
Last month
0
0
controllato il 22-mar-2024
Page view(s)
123
Last Week
0
0
Last month
1
1
controllato il 27-mar-2024
Download(s)
5
controllato il 27-mar-2024
Google ScholarTM
Check
Altmetric
Tutti i documenti nella community "Unitus Open Access" sono pubblicati ad accesso aperto.
Tutti i documenti nella community Prodotti della Ricerca" sono ad accesso riservato salvo diversa indicazione per alcuni documenti specifici