Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2067/42806
Titolo: Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences
Autori: Freitas Leal, Joames K
Lasonder, Edwin
Sharma, Vikram
Schiller, Jürgen
Fanelli, Giuseppina
Rinalducci, Sara 
Brock, Roland
Bosman, Giel
Data pubblicazione: 2020
Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanisms of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, and metabolomic analyses to obtain an integrated understanding of the mechanisms underlying microvesicle generation during the storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells.
URI: http://hdl.handle.net/2067/42806
ISSN: 2227-7382
DOI: 10.3390/proteomes8020006
Diritti: Attribution 3.0 United States
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