Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/42806
Title: | Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences | Authors: | Freitas Leal, Joames K Lasonder, Edwin Sharma, Vikram Schiller, Jürgen Fanelli, Giuseppina Rinalducci, Sara Brock, Roland Bosman, Giel |
Journal: | PROTEOMES | Issue Date: | 2020 | Abstract: | Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanisms of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, and metabolomic analyses to obtain an integrated understanding of the mechanisms underlying microvesicle generation during the storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells. |
URI: | http://hdl.handle.net/2067/42806 | ISSN: | 2227-7382 | DOI: | 10.3390/proteomes8020006 | Rights: | Attribution 3.0 United States |
Appears in Collections: | A1. Articolo in rivista |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Proteomes 2020_RBC microvesicles_Bosman.pdf | 980.29 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
10
12
Last Week
0
0
Last month
1
1
checked on Apr 21, 2024
Page view(s)
96
Last Week
1
1
Last month
1
1
checked on Apr 20, 2024
Download(s)
15
checked on Apr 20, 2024
Google ScholarTM
Check
Altmetric
This item is licensed under a Creative Commons License