Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/42781
Title: | A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy | Authors: | Mazzaglia, Angelo Rahi, Yaseen Jundi Taratufolo, Maria Claudia Tatì, Marta Turco, Silvia Ciarroni, Serena Tagliavento, Vincenzo Valentini, Franco D'Onghia, Anna Maria Balestra, Giorgio Mariano |
Journal: | SCIENTIFIC REPORTS | Issue Date: | 2020 | Abstract: | The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a multiple locus VNTR analysis assay, a molecular tool to differentiate between populations of the pathogen. It has already been successfully applied to different X. fastidiosa subspecies from various plant hosts. The previously published TR loci, together with a set of new design, have been tested in silico on the genome of the Apulian De Donno strain. The resulting selection of 37 TR loci was amplified on the genomic DNAs of the Apulian strains AND from representatives of X. fastidiosa subspecies, and directly on DNA extracted from infected plants. The assay clearly discerned among subspecies or even sequence types (ST), but also pointed out variants within the same ST so as to provide more detailed information on the dynamics and pathogen diffusion pathways. Its effective application even on total DNAs extracted from infected tissues of different host plants makes it particularly useful for large-scale screening of infection and for the strengthening of containment measures. |
URI: | http://hdl.handle.net/2067/42781 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-020-68072-5 | Rights: | Attribution-NonCommercial-NoDerivs 3.0 United States |
Appears in Collections: | A1. Articolo in rivista |
Files in This Item:
File | Description | Size | Format | Existing users please |
---|---|---|---|---|
s41598-020-68072-5.pdf | 1.41 MB | Adobe PDF | Request a copy |
SCOPUSTM
Citations
20
2
Last Week
0
0
Last month
0
0
checked on Apr 21, 2024
Page view(s)
91
Last Week
0
0
Last month
0
0
checked on Apr 20, 2024
Download(s)
4
checked on Apr 20, 2024
Google ScholarTM
Check
Altmetric
This item is licensed under a Creative Commons License