Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/42584
Title: Design and characterization of chionodracine-derived antimicrobial peptides with enhanced activity against drug-resistant human pathogens
Authors: Olivieri, Cristina
Bugli, Francesca
Menchinelli, Giulia
Veglia, Gianluigi
Buonocore, Francesco 
Scapigliati, Giuseppe 
Stocchi, Valentina
Ceccacci, Francesca
Papi, Massimiliano
Sanguinetti, Maurizio
Porcelli, Fernando 
Journal: RSC ADVANCES 
Issue Date: 2018
Abstract: 
© The Royal Society of Chemistry. Starting from the sequence of the amphipathic α-helix of chionodracine (Cnd, 22 amino acids), we designed a series of mutants to increase Cnd's antimicrobial activity and selectivity toward prokaryotic cells and drug-resistant bacterial pathogens. We characterized these new Cnd-derived peptides using fluorescence, CD spectroscopy, and transmission electron microscopy, studying their interactions with synthetic lipid vesicles and assaying their biological function against E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, and Enterobacter sp. Upon interaction with model membranes, these new peptides with higher net charges and hydrophobic moments adopt a helical conformation similar to Cnd. Notably, they display a low cytotoxic activity against human primary cells, a low hemolytic activity, but a significantly high bactericidal activity against drug-resistant bacterial pathogens. The low values of micromolar minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) make these Cnd-derived peptides potential templates to develop antimicrobial agents against drug-resistant human pathogens.
URI: http://hdl.handle.net/2067/42584
ISSN: 2046-2069
DOI: 10.1039/C8RA08065H
Rights: Attribution-NonCommercial-NoDerivs 3.0 United States
Appears in Collections:A1. Articolo in rivista

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