Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/42288
DC FieldValueLanguage
dc.contributor.authorLuziatelli, Francescait
dc.contributor.authorBrunetti, Lorenzait
dc.contributor.authorFicca, Anna Graziait
dc.contributor.authorRuzzi, Maurizioit
dc.date.accessioned2021-01-13T06:21:57Z-
dc.date.available2021-01-13T06:21:57Z-
dc.date.issued2019it
dc.identifier.issn2296-4185it
dc.identifier.urihttp://hdl.handle.net/2067/42288-
dc.description.abstractThe rising demand of bio-vanillin and the possibility to use microbial biotransformation to produce this compound from agroindustrial byproducts are economically attractive. However, there are still several bottlenecks, including substrate and product toxicity, formation of undesired products and genetic stability of the recombinant strains, that impede an efficient use of recombinant Escherichia coli strains to make the whole process cost effective. To overcome these problems, we developed a new E. coli strain, named FR13, carrying the Pseudomonas genes encoding feruloyl-CoA synthetase and feruloyl-CoA hydratase/aldolase integrated into the chromosome and, using resting cells, we demonstrated that the vanillin yield and selectivity were strongly affected by the physiological state of the cells, the temperature used for the growth and the recovery of the biomass and the composition and pH of the bioconversion buffer. The substrate consumption rate and the vanillin yield increased using a sodium/potassium phosphate buffer at pH 9.0 as bioconversion medium. Optimization of the bioprocess variables, using response surface methodology, together with the use of a two-phase (solid-liquid) system for the controlled release of ferulic acid allowed us to increase the vanillin yield up to 28.10 ± 0.05 mM. These findings showed that recombinant plasmid-free E. coli strains are promising candidates for the production of vanillin at industrial scale and that a reduction of the cost of the bioconversion process requires approaches that minimize the toxicity of both ferulic acid and vanillin.it
dc.format.mediumELETTRONICOit
dc.language.isoengit
dc.titleMaximizing the Efficiency of Vanillin Production by Biocatalyst Enhancement and Process Optimizationit
dc.typearticle*
dc.identifier.doi10.3389/fbioe.2019.00279it
dc.identifier.pmid31681753it
dc.identifier.scopus2-s2.0-85074667605it
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fbioe.2019.00279/fullit
dc.relation.journalFRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGYit
dc.relation.firstpage279it
dc.relation.numberofpages14it
dc.relation.volume7it
dc.description.numberofauthors4it
dc.description.internationalnoit
dc.contributor.countryITAit
dc.type.refereeREF_1it
dc.type.miur262*
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.grantfulltextrestricted-
item.openairetypearticle-
item.cerifentitytypePublications-
crisitem.journal.journalissn2296-4185-
crisitem.journal.anceE222470-
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