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http://hdl.handle.net/2067/41018
Titolo: | The face of epicardial and endocardial derived cells in zebrafish | Autori: | Romano, Nicla Ceci, Marcello |
Rivista: | EXPERIMENTAL CELL RESEARCH | Data pubblicazione: | 2018 | Abstract: | Zebrafish hearts can regenerate through activation of growth factors and trans-differentiation of fibroblasts, epicardial, myocardial and endocardial cells, all positive for GATA4 during the process. A possible model of regeneration of the whole heart and the regenerating cells in ex-vivo culture is presented here by a stimulation of cocktail of growth factors. In ex-vivo growth-factors-supplemented culture the heart regeneration was quite complete without signs of fibrosis. Epicardial- and endocardial-derived cells have been analyzed by electron microscopy evidencing two main types: 1) larger/prismatic and 2) small/rounded. Type (1) showed on the surface protein-sculptures, while type(2) was smooth with sparse globular proteins. To confirm their nature we have contemporarily analyzed their proliferative capability and markers-positivity. The cells treated by growth factors have at least two-fold more proliferation with GATA4-positivity. The type (1) cell evidenced WT1+(marker of embryonic epicardium); the type (2) showed NFTA2+(marker of embryonic endocardium); whereas cTNT-cardiotroponin was negative. Under growth factors stimulation, GATA4+/WT1+ and GATA4+/ NFTA2+ could be suitable candidates to be the cells with capability to move in/out of the tissue, probably by using their integrins, and it opens the possibility to have long term selected culture to future characterization. |
URI: | http://hdl.handle.net/2067/41018 | ISSN: | 00144827 | DOI: | 10.1016/j.yexcr.2018.05.022 |
È visualizzato nelle collezioni: | A1. Articolo in rivista |
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