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Title: The face of epicardial and endocardial derived cells in zebrafish
Authors: Romano, Nicla 
Ceci, Marcello 
Issue Date: 2018
Zebrafish hearts can regenerate through activation of growth factors and trans-differentiation of fibroblasts,
epicardial, myocardial and endocardial cells, all positive for GATA4 during the process. A possible model of
regeneration of the whole heart and the regenerating cells in ex-vivo culture is presented here by a stimulation of
cocktail of growth factors. In ex-vivo growth-factors-supplemented culture the heart regeneration was quite
complete without signs of fibrosis. Epicardial- and endocardial-derived cells have been analyzed by electron
microscopy evidencing two main types: 1) larger/prismatic and 2) small/rounded. Type (1) showed on the
surface protein-sculptures, while type(2) was smooth with sparse globular proteins. To confirm their nature we
have contemporarily analyzed their proliferative capability and markers-positivity. The cells treated by growth
factors have at least two-fold more proliferation with GATA4-positivity. The type (1) cell evidenced
WT1+(marker of embryonic epicardium); the type (2) showed NFTA2+(marker of embryonic endocardium);
whereas cTNT-cardiotroponin was negative. Under growth factors stimulation, GATA4+/WT1+ and GATA4+/
NFTA2+ could be suitable candidates to be the cells with capability to move in/out of the tissue, probably by
using their integrins, and it opens the possibility to have long term selected culture to future characterization.
ISSN: 00144827
DOI: 10.1016/j.yexcr.2018.05.022
Appears in Collections:A1. Articolo in rivista

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