Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/38473
Title: Phenotypic, genetic and molecular characterization of 7B-1, a conditional male-sterile mutant in tomato
Authors: Pucci, A.
Picarella Me
Mazzucato, Andrea 
Journal: THEORETICAL AND APPLIED GENETICS 
Issue Date: 2017
Abstract: 
The interest in male sterility (MS) dates back to
a long time due to its perspective use in hybrid seed production.
Here, we characterize 7B-1, a photoperiod-sensitive
male-sterile (ms) mutant in tomato (Solanum lycopersicum
L.), in which stamens are restored to fertility by permissive
growth conditions in short days (SD). This system represents
a useful strategy to facilitate the maintenance of the ms line.
Examination of 7B-1 and other structural mutants, vms, sl,
sl-2 and tap3, showed carpellization of stamens in the third
floral whorl. 7B-1 exhibits strong expressivity in long days
(LD), producing 100% aberrant anthers and virtually no seed
production under open pollination, whereas it recovered fertility
in SD. By genetic analysis, we demonstrate that 7B-1 is
not allelic to sl nor to vms; instead it shows allelism to sl-2.
Because the homeotic phenotype of the mutation resembles
lesions to members of the B-class MADS-box transcription
factor family, that specify petal and stamen identity, wepursued a candidate gene approach towards these targets.
Using an interspecific backcross mapping population and
markers linked to B-class MADS-box genes, significant
linkage was found between 7B-1 and the SlGLO2 gene on
Chr6. This result was supported by the 7B-1 phenotype that
is similar to that of SlGLO2 knock outs and by the strong
downregulation of the gene in the mutant. Although the
lesion underlying the mutant phenotype is still elusive, our
results pave the way for the final demonstration that SlGLO2
underlies 7B-1 and further the use of 7B-1 mutant in tomato
hybrid seed production schemes.
URI: http://hdl.handle.net/2067/38473
ISSN: 0040-5752
DOI: 10.1007/s00122-017-2964-7
Appears in Collections:A1. Articolo in rivista

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