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Title: Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells
Authors: Basiricò, Loredana 
Morera, P. 
Dipasquale, D.
Tröscher, A.
Bernabucci, Umberto 
Issue Date: 2017
Some in vitro and in vivo studies have demonstrated
protective effects of conjugated linoleic acid (CLA)
isomers against oxidative stress and lipid peroxidation.
However, only a few and conflicting studies have been
conducted showing the antioxidant potential of essential fatty acids. The objectives of the study were to
compare the effects of CLA to other essential fatty acids
on the thiol redox status of bovine mammary epithelia
cells (BME-UV1) and their protective role against oxidative damage on the mammary gland by an in vitro
study. The BME-UV1 cells were treated with complete
medium containing 50 µM of cis-9,trans-11 CLA,
trans-10,cis-12 CLA, α-linolenic acid, γ-linolenic acid,
and linoleic acid. To assess the cellular antioxidant response, glutathione, NADPH, and γ-glutamyl-cysteine
ligase activity were measured 48 h after addition of
fatty acids (FA). Intracellular reactive oxygen species
and malondialdehyde production were also assessed in
cells supplemented with FA. Reactive oxygen species
production after 3 h of H2O2 exposure was assessed to
evaluate and to compare the potential protection of
different FA against H2O2-induced oxidative stress. All
FA treatments induced an intracellular GSH increase,
matched by high concentrations of NADPH and an
increase of γ-glutamyl-cysteine ligase activity. Cells
supplemented with FA showed a reduction in intracellular malondialdehyde levels. In particular, CLA isomers and linoleic acid supplementation showed a better
antioxidant cellular response against oxidative damage
induced by H2O2 compared with other FA.
ISSN: 0022-0302
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Appears in Collections:A1. Articolo in rivista

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