Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/2184
Title: HRM technology for the identification and characterization of INDEL and SNPs mutations in genes involved in drought and salt tolerance of durum wheat
Authors: Mondini, Linda
Nachit, Miloudi
Porceddu, Enrico
Pagnotta, Mario Augusto
Keywords: Durum wheat;High-resolution melting;Salt tolerance;Single-nucleotide polymorphisms
Issue Date: 2011
Publisher: Cambridge University Press
Source: Mondini, L. et al. 2011. HRM technology for the identification and characterization of INDEL and SNPs mutations in genes involved in drought and salt tolerance of durum wheat. "Plant Genetic Resources" 9: 166-169
Abstract: 
WRKY transcription factors are one of the largest families of transcriptional regulators and form an integral part of signalling webs which modulate many plant processes, such as abiotic stress tolerance. In the present paper, an innovative method has been applied to identify novel WRKY-1 alleles involved in the responses to salt and drought stresses in Triticum durum.
This technique involves scanning for sequencing variations in cDNA-derived PCR amplicons, using high-resolution melting (HRM) followed by direct Sanger sequencing of only those amplicons which were predicted to carry nucleotide changes. HRM represents a novel advance in detection of single-nucleotide polymorphisms (SNPs) by measuring temperature-induced strand separation of short PCR amplicons. The use of this approach is still limited in the field of plant biology. Here, HRM analysis has been applied to the discovery and genotyping of durum wheat SNPs. Specific primers have been designed, starting at multi-alignment of
WRKY-1-conserved portions. The PCR amplicons, containing single SNPs, produce distinctive HRM profiles, and by sequencing the PCR products identified, SNPs have been characterized and validated. The results showed that all the revealed SNPs are located on salt-tolerant varieties, confirming their value in breeding activities.
Description: 
L'articolo è disponibile sul sito dell'editore http://www.cambridge.org
URI: http://hdl.handle.net/2067/2184
ISSN: 1479-2621
DOI: 10.1017/S1479262111000517
Appears in Collections:DABAC - Archivio della produzione scientifica

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