Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/2183
Title: Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies
Authors: Picchietti, Simona
Guerra, Laura
Bertoni, Fabrizio
Randelli, Elisa
Belardinelli, Maria Cristina
Buonocore, Francesco
Rombout, Jan
Scapigliati, Giuseppe
Abelli, Luigi
Keywords: Fish Immunology;Evolution of immune system;GALT;Thymus;RNA in situ hybridization;Real-time PCR;Cytotoxic T cells;Dicentrarchus labrax
Issue Date: 2011
Publisher: Elsevier
Source: Picchietti, S. et al. 2011. Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies. "Fish & Shellfish Immunology" 30 (2): 609-617
Abstract: 
Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-α, MHCI-α, MHCII-β, rag-1 and TCR-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.).
Real-time PCR findings depicted a main role of the thymus in T cell development, but also rag-1 and CD8-α transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-β and CD8-α exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-α+ cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T cell-specific gene expressions are first described in the intestine of a bony fish.
High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles.
Description: 
L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/
URI: http://hdl.handle.net/2067/2183
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2010.12.006
Appears in Collections:DISA - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
IntTcells.doc30 kBMicrosoft WordView/Open
Show full item record

Page view(s)

5
Last Week
0
Last month
4
checked on Nov 25, 2020

Download(s)

5
checked on Nov 25, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.