Please use this identifier to cite or link to this item:
Title: Zinc deficiency suppresses the development of oral tolerance in rats
Authors: Finamore, Alberto
Roselli, Marianna
Merendino, Nicolò 
Nobili, Fabio
Vignolini, Francesco
Mengheri, Elena
Keywords: Zinc deficiency;Oral tolerance;Intestine;Cytokines;Rats
Issue Date: 2003
Publisher: American Society for Nutritional Sciences
Source: Finamore, A. et al. 2003. Zinc deficiency suppresses the development of oral tolerance in rats. "Journal of nutrition" 133 (1): 191-198
Oral tolerance is a specific immune unresponsiveness to food antigens to prevent hypersensitivity
reactions. We investigated whether zinc deficiency affects oral tolerance. Rats were fed a control (C) or zincdeficient (ZD) diet, or pair-fed (PF) to ZD rats for 28 d. Beginning on d 7, rats were administered ovalbumin (OVA)
orally to induce tolerance, or PBS 3 times/wk, and were then immunized by OVA injection. The proliferation of
mesenteric lymph node (MLN) and spleen lymphocytes after in vitro OVA stimulation and the delayed-type
hypersensitivity were higher in OVA-fed ZD than in OVA-fed C rats and not different between OVA- and PBS-fed
ZD rats, indicating a suppression of tolerance. Lymphocyte proliferation did not differ between PF and C rats.
Expressions of cytokines involved in oral tolerance, i.e., interleukin (IL)-4, IL-10 and transforming growth factor- ,
were higher in OVA- than in PBS-fed C rats, but not in ZD rats. Apoptosis was higher in OVA- than in PBS-fed C
rats but not different between OVA- and PBS-fed ZD rats. Inflammation and ulcerations that were not present in
ZD rats on d 7 (ZD7
) developed in OVA- or PBS-fed ZD rats. Compared with ZD7
rats, tumor necrosis factor- and
cytokine-induced neutrophil chemoattractant were higher in OVA- and PBS-fed ZD rats, whereas interferon-
increased only in OVA-fed ZD rats. In conclusion, zinc deficiency suppresses oral tolerance through dysregulation
of cytokine expression and lack of antigen-specific clonal deletion. We suggest that abrogation of tolerance may
lead to development of mucosal inflammation and damage.
L'articolo è disponibile sul sito dell'editore
ISSN: 0022-3166
Appears in Collections:DECOS - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
JNutrition2069.pdf106.3 kBAdobe PDFView/Open
Show full item record

Page view(s)

Last Week
Last month
checked on Jul 13, 2024


checked on Jul 13, 2024

Google ScholarTM


All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents