Fatty acids affect proliferation of peripheral blood mononuclear cells in dairy cows

Abstract

In vitro studies were performed to assess the effects of bovine plasma fatty acids on proliferation of peripheral blood mononuclear cells (PBMC). PBMC from 6 Holstein heifers were cultured in media containing oleic (OA), palmitic (PA), stearic (SA), linoleic (LA), palmitoleic (POA), or linolenic (LNA) acid at concentrations mimicking different degree of lipomobilisation. Proliferation of PBMC was stimulated by concanavalin A or pokeweed mitogen. Concentrations of OA, PA, SA and LA mimicking moderate-intense lipomobilisation impaired PBMC proliferation. Concentrations of OA or LA mimicking low degree of lipomobilisation enhanced PBMC proliferation. None of the POA, and LNA concentrations affected proliferation of PBMC.