The parthenocarpic fruit (pat) mutant of tomato (Lycopersicon esculentum Mill.) sets seedless fruits and has aberrant anther and ovule development

Abstract

Among the different sources of genetic parthenocarpy described in tomato, the mutation referred to as parthenocarpic fruit (pat) is of particular interest because of its strong expressivity and because it confers better earliness, higher fruit set and enhanced fruit quality. As a pleiotropic effect, pat flowers have aberrantly developing androecia and reduced male and female fertility. In this work we extend the early description of the pat phenotype by investigating the expression of parthenocarpy in three different environments and by using light and scanning electron microscopy to analyse the development of male and female floral organs. The degree of parthenocarpy was high in the three experimental environments and was characterised by an initiation of ovary growth anticipated to pre-anthesis floral stages. Aberrations in anther development were evident at flower bud stages and resulted in shorter, irregular and teratoid organs. Ectopic production of carpel-like structures bearing external ovules was evident in the most severely altered androecia. Analysis of ovule development revealed that a fraction of pat ovules becomes aberrant from very early stages, having defective integument growth. Meiosis was irregular in aberrant ovules and megaspores or gametes production was severely hampered. The described pat syndrome suggests that parthenocarpy in this mutant could be a secondary effect of a gene controlling, at early stages, organ identity and development.