Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/1835
DC Field | Value | Language |
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dc.contributor.author | Gennaro, Andrea | - |
dc.contributor.author | Koebner, Robert M.D. | - |
dc.contributor.author | Ceoloni, Carla | - |
dc.date.accessioned | 2011-03-31T14:15:18Z | - |
dc.date.available | 2011-03-31T14:15:18Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Gennaro A., Koebner R.M.D., Ceoloni C. 2009. A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat. "Functional and Integrative Genomics" 9(3): 325-334. | en |
dc.identifier.issn | 1438-793X | - |
dc.identifier.uri | http://hdl.handle.net/2067/1835 | - |
dc.description | L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com. | it |
dc.description.abstract | Lr19, one of the few widely effective genes conferring resistance to leaf-rust in wheat, was transferred from the wild relative Thinopyrum ponticum to durum wheat. Since Lr19 confers a hypersensitive response to the pathogen, it was considered likely that the gene would be a member of the major nucleotide-binding site (NBS)-leucine-rich repeat (LRR) plant R gene family. NBS profiling, based on PCR amplification of conserved NBS motifs, was applied to durum wheat-Thinopyrum ponticum recombinant lines involving different segments of the alien 7AgL chromosome arm, carrying or lacking Lr19. One sequence (AG15), tightly linked to Lr19, a 4,121 bp full-length cDNA was obtained. Quantitative PCR on leaf-rust infected and non-infected Lr19 carriers proved AG15 to be constitutively expressed, as is common for R genes. | en |
dc.description.abstract | L’Lr19, uno dei geni più efficaci nel conferire resistenza alla ruggine bruna del frumento, è stato trasferito dalla specie selvatica Thinopyrum ponticum al frumento duro. In considerazione del fatto che il gene Lr19 determina nell’ostite una risposta di tipo ipersensibile, è probabile che esso appartenga alla principale famiglia di geni di resistenza (R), ovvero gli NBS-LRR. L’NBS-profiling, una tecnica basata sull’amplificazione PCR dei motivi NBS più conservati, è stata applicata su linee ricombinanti frumento duro-Thinopyrum ponticum portatrici e non del gene Lr19. E’ stata isolata una sequenza completa (AG15) di cDNA lunga 4.121 bp, strettamente associata all’Lr19. Eseguendo una PCR quntitativa su linee portatrici dell’Lr19, sottoposte e non, ad infezione con ruggine bruna, si è evidenziato come la sequenza AG15 sia costitutivamente espressa, come atteso per i geni di tipo R. | it |
dc.language.iso | en | it |
dc.publisher | Springer-Verlag | it |
dc.subject | Triticum durum | it |
dc.subject | Thinopyrum ponticum | it |
dc.subject | NBS-LRR genes | it |
dc.subject | Geni NBS-LRR | it |
dc.subject | RGAs | it |
dc.subject | Lr19 | it |
dc.subject | Codominant marker | it |
dc.subject | Marcatore codominante | it |
dc.title | A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat | it |
dc.type | Article | it |
dc.identifier.doi | 10.1007/s10142-009-0115-1 | - |
item.fulltext | With Fulltext | - |
item.openairetype | Article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | DABAC - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
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Gennaro_et_al_FIG_2009.pdf | 16.97 kB | Adobe PDF | View/Open |
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