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http://hdl.handle.net/2067/1828
Title: | Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations | Authors: | Mendoza-de Gyves, Emilio Royani, Juwartina Ida Rugini, Eddo |
Keywords: | Plant growth regulators;Putrescine;Rooting;Nodal explant;Giberellic acid;Fitoregolatori;Putrescina;Radicazione;Espianto nodale;Acido gibberellico | Issue Date: | 2007 | Publisher: | EDP Sciences | Source: | Mendoza-de Gyves E., Royani J. I., Rugini E. 2007. Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations. "Annals of Forest Science" 64(1): 73-78 | Abstract: | Multiple shoots of high quality were produced in vitro from nodal explants of Tectona grandis. An average of about 4 shoots/uninodal explant was obtained within 4 weeks of culture on Murashige and Skoog’s (mMS) medium modified by 50% reduction in NH4NO3 concentration, supplemented with benzylaminopurine (1.5 mg L−1); indole-3-butyric acid (0.01 mg L−1) and gibberellic acid (0.1 mg L−1). The latter was applied bothin the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L−1. Hundred percent of shoots rooted cultured onmodified MS medium containing IBA (0.5 mg L−1) and putrescine (160 mg L−1). Putrescine promoted both strong and highly ramified roots and fastgrowing shoots during the rooting phase, conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a shortacclimatization stage in greenhouse where they survived at 100%. This highly reproducible procedure can be adopted for large scale teak propagation. |
Description: | L'articolo è disponibile sul sito dell'editore http://www.edpsciences.org |
URI: | http://hdl.handle.net/2067/1828 | ISSN: | 1286-4560 | DOI: | 10.1051/forest:2006090 |
Appears in Collections: | DIPROV - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
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Mendoza et al. 2007.pdf | 854.15 kB | Adobe PDF | View/Open |
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