Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1802
Title: Heat shock impairs DNA synthesis and down-regulates gene expression for leptin and Ob-Rb receptor in concanavalin A-stimulated bovine peripheral blood mononuclear cells
Authors: Lacetera, Nicola
Bernabucci, Umberto
Basiricò, Loredana
Morera, Patrizia
Nardone, Alessandro
Keywords: Heat stress;Stress da caldo;Gene expression;Espressione genica;Leptin;Leptina;Lymphocytes;Linfociti
Issue Date: 2009
Publisher: Elsevier
Source: Lacetera, N. et al. 2009. Heat shock impairs DNA synthesis and down-regulates gene expression for leptin and Ob-Rb receptor in concanavalin A-stimulated bovine peripheral blood mononuclear cells. "Veterinary Immunology and Immunopathology" 127: 190-194
Abstract: 
This study verified whether leptin or its long isoform receptor (Ob-Rb) genes are expressed in proliferating lymphocytes from bovine species, and whether their expression
changes with increased temperatures. Peripheral blood mononuclear cells (PBMC)
from five Holstein cows were incubated in the presence of concanavalin A, and alternatively subjected for 65 h to each of the following treatments (T): 39 8C continuously (T39) or three 13-h cycles at 40 (T40), 41 (T41) or 42 8C (T42), respectively, which were alternated with two 13-h cycles at 39 8C. T39 mimicked normothermia; T40, 41 and 42 mimicked conditions of hyperthermia alternated with normothermia. PBMC proliferation declined under T42. Compared with T39, levels of mRNA for leptin was lower under T42, whereas mRNA for Ob-Rb was lower in lymphocytes cultured both under T41 and T42. DNA synthesis was positively correlated with leptinmRNA. This study supports the concept that severe heat stress impairs proliferation of bovine PBMC, confirms that bovine lymphocytes express Ob-Rb gene, and provides the first experimental evidence that bovine lymphocytes express gene for leptin, and that increased temperatures are associated with altered gene expression for leptin and Ob-Rb.
Description: 
L’articolo è disponibile sul sito dell’editore http://www.sciencedirect.com/science/journal/01652427
URI: http://hdl.handle.net/2067/1802
ISSN: 0165-2427
DOI: 10.1016/j.vetimm.2008.09.020
Appears in Collections:DiPA - Archivio della produzione scientifica

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