Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/1784
Title: | Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress. | Authors: | Laino, Paolo Shelton, Dale Finnie, Christine De Leonardis, Anna Maria Mastrangelo, Anna Maria Svensson, Birte Lafiandra, Domenico Masci, Stefania |
Keywords: | Durum wheat;Heat stress;Proteome;Wheat kernel;Metabolic proteins | Issue Date: | 2010 | Publisher: | Wiley-Blackwell | Source: | Laino, P. et al. 2010. Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress. "Proteomics" 10(12):2359-2368 | Abstract: | In Central and Southern Italy, where durum wheat represents one of the most widely cultivated crops, grain filling occurs during Spring, a period characterised by sudden increases in temperature. Wheat grain proteins are classified into albumins, globulins, and prolamins. The non-prolamin fractions include proteins with metabolic activity or structural function. In order to investigate the consequences of heat stress on the accumulation of non-prolamin proteins in mature durum wheat kernels, the Italian cultivar Svevo was subjected to two thermal regimes (heat stress vs. control) during grain filling. The 2D patterns of non-prolamin proteins were monitored to identify polypeptides affected by heat stress. This study shows that heat stress alters significantly the durum wheat seed proteome, although the fold changes range only between 1.2 and 2.2. This analysis revealed 132 differentially expressed polypeptides, 47 of which were identified by MALDI TOF and MALDI-TOF-TOF MS and included heat shock proteins, proteins involved in the glycolysis and carbohydrate metabolism, as well as stress related proteins. Many of the heat induced polypeptides are considered to be allergenic for sensitive individuals. The differences observed with previously reported data regarding bread wheat may be explained by the absence of the D genome in durum wheat |
Description: | L'articolo è disponibile sul sito dell'editore: www.interscience.wiley.com |
URI: | http://hdl.handle.net/2067/1784 | ISSN: | 1615-9853 | DOI: | 10.1002/pmic.200900803 |
Appears in Collections: | DABAC - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
submitted version 1.pdf | 729.08 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
1
101
Last Week
0
0
Last month
0
0
checked on Sep 18, 2023
Page view(s)
82
Last Week
0
0
Last month
0
0
checked on Mar 16, 2024
Download(s)
267
checked on Mar 16, 2024
Google ScholarTM
Check
Altmetric
All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents